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1.
Front Immunol ; 12: 713799, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34539643

RESUMO

Rheumatoid arthritis (RA) is significantly associated with glycolysis. This study used 2-deoxy-D-glucose (2-DG), an inhibitor of glycolysis, to treat rats with collagen-induced arthritis (CIA) and investigate the metabolic regulatory mechanism of glycolysis in the disease. 2-DG significantly alleviated CIA. Metabolomics and transcriptomics, as well as their integrative analysis, detected significant changes in the pathways of bile secretion, cholesterol and linoleic acid metabolism in the plasma, liver and spleen during the CIA process and the opposite changes following 2-DG treatment, whereas the expression of the genes regulating these metabolic pathways were changed only in the spleen. In the rat liver, levels of (S)-5-diphosphomevalonic acid in the terpenoid backbone biosynthesis pathway were significantly decreased during CIA progression and increased following 2-DG treatment, and levels of taurochenodeoxycholic acid in the pentose and glucuronate interconversions pathway showed the opposite results. In the spleen, levels of 3-methoxy-4-hydroxyphenylglycol glucuronide in bile secretion and 12(S)-leukotriene B4 in arachidonic acid metabolism were significantly decreased during CIA progression and increased following 2-DG treatment. The changes in the gene-metabolite network of bile secretion in the spleen correlated with a decreased plasma L-acetylcarnitine level in CIA rats and an increase following 2-DG treatment. Our analysis suggests the involvement of spleen and liver metabolism in CIA under the control of glycolysis.


Assuntos
Artrite Experimental/etiologia , Artrite Experimental/metabolismo , Metabolismo Energético , Glucose/metabolismo , Fígado/imunologia , Fígado/metabolismo , Baço/imunologia , Baço/metabolismo , Animais , Artrite Experimental/patologia , Biologia Computacional/métodos , Citocinas/metabolismo , Perfilação da Expressão Gênica , Glicólise , Fígado/patologia , Contagem de Linfócitos , Subpopulações de Linfócitos/imunologia , Subpopulações de Linfócitos/metabolismo , Subpopulações de Linfócitos/patologia , Metabolômica/métodos , Ratos , Baço/patologia
2.
Ann Hematol ; 100(11): 2727-2732, 2021 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-34331563

RESUMO

Treatment options for relapsed or refractory B-lymphoblastic leukaemia (r/r B-ALL) are limited and the prognosis of these patients remains dismal, but novel immunotherapeutic options such as the anti-CD22 antibody-drug-conjugate Inotuzumab-Ozogamicin (InO) have improved outcomes in these patients. Flow cytometry is essential to assess antigen-expression prior to treatment initiation of antigen-directed immunotherapies. Here, we present flow cytometric and clinical data of three adult patients with r/r B-ALL who failed treatment with InO associated with reduced or lost antigen-expression. In addition, we present comparative data on two different diagnostic CD22-specific antibody clones that exhibit significant differences in staining intensities.


Assuntos
Antineoplásicos Imunológicos/uso terapêutico , Linfócitos B/química , Inotuzumab Ozogamicina/uso terapêutico , Subpopulações de Linfócitos/química , Leucemia-Linfoma Linfoblástico de Células Precursoras B/tratamento farmacológico , Lectina 2 Semelhante a Ig de Ligação ao Ácido Siálico/análise , Adulto , Idoso de 80 Anos ou mais , Aloenxertos , Anticorpos Biespecíficos/uso terapêutico , Protocolos de Quimioterapia Combinada Antineoplásica/uso terapêutico , Linfócitos B/patologia , Células Clonais , Feminino , Citometria de Fluxo , Transplante de Células-Tronco Hematopoéticas , Humanos , Mesilato de Imatinib/administração & dosagem , Imunofenotipagem , Subpopulações de Linfócitos/patologia , Masculino , Pessoa de Meia-Idade , Leucemia-Linfoma Linfoblástico de Células Precursoras B/sangue , Leucemia-Linfoma Linfoblástico de Células Precursoras B/patologia , Recidiva , Terapia de Salvação , Sorafenibe/uso terapêutico , Falha de Tratamento , Adulto Jovem
3.
Bull Exp Biol Med ; 166(2): 245-249, 2018 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-30488207

RESUMO

The morphometric analysis of mesenteric lymph nodes was carried out in female Wistar rats with chemically induced breast cancer. In control rats with untreated breast cancer, the volume of the system of sinuses increased in parallel with the appearance of morphological signs of suppression of cell-mediated immunity, inhibition of humoral immunity, and macrophage reaction. Against the background of chemotherapy, we observed a decrease in the volume of paracortex and lymphoid nodules, suppression of proliferative activity of lymphoid cells in paracortical and B-cell zone, and a decrease in macrophage content. After resection of breast cancer followed by chemotherapy course, lymph transport activation, widening of the paracortex, enhanced proliferative activity of cells in the paracortex and B-cell zone, and reduced volumes of lymphoid nodules with and without germinal centers and medullary substance were revealed in comparison with rats subjected neoadjuvant chemotherapy.


Assuntos
Protocolos de Quimioterapia Combinada Antineoplásica/farmacologia , Linfonodos/efeitos dos fármacos , Subpopulações de Linfócitos/efeitos dos fármacos , Neoplasias Mamárias Experimentais/tratamento farmacológico , Terapia Neoadjuvante/métodos , Animais , Ciclofosfamida/farmacologia , Feminino , Fluoruracila/farmacologia , Linfonodos/imunologia , Linfonodos/patologia , Subpopulações de Linfócitos/imunologia , Subpopulações de Linfócitos/patologia , Macrófagos/efeitos dos fármacos , Macrófagos/imunologia , Macrófagos/patologia , Glândulas Mamárias Animais/efeitos dos fármacos , Glândulas Mamárias Animais/imunologia , Glândulas Mamárias Animais/patologia , Neoplasias Mamárias Experimentais/induzido quimicamente , Neoplasias Mamárias Experimentais/imunologia , Neoplasias Mamárias Experimentais/patologia , Mastócitos/efeitos dos fármacos , Mastócitos/imunologia , Mastócitos/patologia , Mesentério , Metotrexato/farmacologia , Metilnitrosoureia , Neutrófilos/efeitos dos fármacos , Neutrófilos/imunologia , Neutrófilos/patologia , Ratos , Ratos Wistar
4.
Clin Exp Immunol ; 173(1): 67-75, 2013 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-23607771

RESUMO

Sjögren's syndrome is a chronic illness manifested characteristically by immune injury to the salivary and lacrimal glands, resulting in dry mouth/eyes. Anti-Ro [Sjögren's syndrome antigen A (SSA)] and anti-La [Sjögren's syndrome antigen B (SSB)] autoantibodies are found frequently in Sjögren's subjects as well as in individuals who will go on to develop the disease. Immunization of BALB/c mice with Ro60 peptides results in epitope spreading with anti-Ro and anti-La along with lymphocyte infiltration of salivary glands similar to human Sjögren's. In addition, these animals have poor salivary function/low saliva volume. In this study, we examined whether Ro-peptide immunization produces a Sjögren's-like illness in other strains of mice. BALB/c, DBA-2, PL/J, SJL/J and C57BL/6 mice were immunized with Ro60 peptide-274. Sera from these mice were studied by immunoblot and enzyme-linked immunosorbent assay for autoantibodies. Timed salivary flow was determined after pharmacological stimulation, and salivary glands were examined pathologically. We found that SJL/J mice had no immune response to the peptide from Ro60, while C57BL/6 mice produced antibodies that bound the peptide but had no epitope spreading. PL/J mice had epitope spreading to other structures of Ro60 as well as to La, but like C57BL/6 and SJL/J had no salivary gland lymphocytic infiltration and no decrement of salivary function. DBA-2 and BALB/c mice had infiltration but only BALB/c had decreased salivary function. The immunological processes leading to a Sjögren's-like illness after Ro-peptide immunization were interrupted in a stepwise fashion in these differing mice strains. These data suggest that this is a model of preclinical disease with genetic control for epitope spreading, lymphocytic infiltration and glandular dysfunction.


Assuntos
Anticorpos Antinucleares/biossíntese , Autoantígenos/imunologia , Autoimunidade/imunologia , Modelos Animais de Doenças , Camundongos Endogâmicos/imunologia , RNA Citoplasmático Pequeno/imunologia , Ribonucleoproteínas/imunologia , Síndrome de Sjogren/imunologia , Sequência de Aminoácidos , Animais , Anticorpos Antinucleares/imunologia , Autoimunidade/genética , Carbacol/farmacologia , Epitopos/imunologia , Adjuvante de Freund , Antígenos H-2/genética , Antígenos H-2/imunologia , Haplótipos , Imunização , Subpopulações de Linfócitos/imunologia , Subpopulações de Linfócitos/patologia , Masculino , Camundongos , Camundongos Endogâmicos/genética , Dados de Sequência Molecular , Músculo Liso/efeitos dos fármacos , Músculo Liso/imunologia , Fragmentos de Peptídeos/imunologia , Sintomas Prodrômicos , Receptor Muscarínico M3/efeitos dos fármacos , Receptor Muscarínico M3/imunologia , Glândulas Salivares/patologia , Salivação , Síndrome de Sjogren/etiologia , Organismos Livres de Patógenos Específicos , Bexiga Urinária , Xerostomia/etiologia , Xerostomia/imunologia , Antígeno SS-B
5.
Clin Endocrinol (Oxf) ; 78(4): 621-8, 2013 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-22957689

RESUMO

CONTEXT: One of the side effects of interferon-alpha therapy is interferon-induced thyroiditis (IIT). The role of lymphocyte subpopulations in IIT melanoma patients remains to be defined. OBJECTIVE: Our objective was to assess different peripheral blood lymphocyte subpopulations, mainly regulatory T cells (Tregs), in melanoma patients who developed IIT. DESIGN, PATIENTS AND METHODS: From 30 melanoma patients receiving high-dose interferon (HDI)-alpha 2b (IFN-α2b) treatment, those who developed IIT (IIT patients) were selected and compared with patients who did not develop IIT (Co-MM) and healthy controls (Co-H). Peripheral blood mononuclear cells were obtained before treatment (BT), mid-treatment (MT), end of treatment (ET), 24 weeks post-treatment and at appearance of IIT (TT). RESULTS: Nine patients developed IIT (30%): four Hashimoto's thyroiditis and five destructive thyroiditis. An increase in Tregs was observed in both melanoma groups during HDI treatment. A decrease in CD3(+) , NKT lymphocyte subpopulations and Bcl2 expression on B cells was also observed in both groups. However, no changes were observed in the percentage of CD4(+) , CD8(+) , CD3(+) γδ(+) , CD19(+) , transitional B cells (CD24(high) CD38(high) CD19(+) CD27(-) ), natural killer (NK), invariant NKT (iNKT) lymphocytes and Th1/Th2 balance when BT was compared with ET. At TT, IIT patients had a higher Tregs percentage than Co-MM (P = 0·012) and Co-H (P = 0·004), a higher iNKT percentage than Co-MM (P = 0·011), a higher transitional B cells percentage than Co-H (P = 0·015), a lower CD3(+) percentage than Co-H (P = 0·001) and a lower Bcl2 expression on B cells than Co-H (P < 0·001). CONCLUSIONS: Our results point to the immunomodulatory effects of IFN-α on different lymphocyte subpopulations and a possible role of Tregs in melanoma patients who developed IIT.


Assuntos
Interferon-alfa/efeitos adversos , Subpopulações de Linfócitos/patologia , Melanoma/imunologia , Neoplasias Cutâneas/imunologia , Linfócitos T Reguladores/patologia , Tireoidite/induzido quimicamente , Adulto , Antivirais/efeitos adversos , Antivirais/uso terapêutico , Feminino , Hepatite C/complicações , Hepatite C/tratamento farmacológico , Hepatite C/imunologia , Humanos , Interferon alfa-2 , Interferon-alfa/administração & dosagem , Interferon-alfa/uso terapêutico , Masculino , Melanoma/complicações , Melanoma/tratamento farmacológico , Melanoma/patologia , Pessoa de Meia-Idade , Proteínas Recombinantes/administração & dosagem , Proteínas Recombinantes/efeitos adversos , Proteínas Recombinantes/uso terapêutico , Neoplasias Cutâneas/complicações , Neoplasias Cutâneas/tratamento farmacológico , Neoplasias Cutâneas/patologia , Testes de Função Tireóidea , Tireoidite/imunologia , Tireoidite/patologia , Adulto Jovem
7.
Neuroscience ; 169(1): 370-7, 2010 Aug 11.
Artigo em Inglês | MEDLINE | ID: mdl-20451583

RESUMO

Experimental autoimmune neuritis (EAN) is a T cell-mediated autoimmune inflammatory demyelinating disease of the peripheral nervous system and serves as the animal model of human inflammatory demyelinating polyradiculoneuropathies. MS-275, a potent histone deacetylase inhibitor currently undergoing clinical investigations for various malignancies, has been reported to demonstrate promising anti-inflammatory activities. In our present study, MS-275 administration (3.5 mg/kg i.p.) to EAN rats once daily from the appearance of first neurological signs greatly reduced the severity and duration of EAN and attenuated local accumulation of macrophages, T cells and B cells, and demyelination of sciatic nerves. Further, significant reduction of mRNA levels of pro-inflammatory interleukin-1beta, interferon-gamma, interleukine-17, inducible nitric oxide synthase and matrix metalloproteinase-9 was observed in sciatic nerves of MS-275 treated EAN rats. In lymph nodes, MS-275 depressed pro-inflammatory cytokines as well, but increased expression of anti-inflammatory cytokine interleukine-10 and of foxhead box protein3 (Foxp3), a unique transcription factor of regulatory T cells. In addition, MS-275 treatment increased proportion of infiltrated Foxp3(+) cells and anti-inflammatory M2 macrophages in sciatic nerves of EAN rats. In summary, our data demonstrated that MS-275 could effectively suppress inflammation in EAN, through suppressing inflammatory T cells, macrophages and cytokines, and inducing anti-inflammatory immune cells and molecules, suggesting MS-275 as a potent candidate for treatment of autoimmune neuropathies.


Assuntos
Anti-Inflamatórios não Esteroides/uso terapêutico , Benzamidas/uso terapêutico , Inibidores de Histona Desacetilases/uso terapêutico , Neurite Autoimune Experimental/tratamento farmacológico , Piridinas/uso terapêutico , Animais , Anti-Inflamatórios não Esteroides/farmacologia , Benzamidas/farmacologia , Citocinas/biossíntese , Citocinas/genética , Doenças Desmielinizantes/patologia , Avaliação Pré-Clínica de Medicamentos , Fatores de Transcrição Forkhead/análise , Regulação da Expressão Gênica/efeitos dos fármacos , Inibidores de Histona Desacetilases/farmacologia , Inflamação , Linfonodos/metabolismo , Linfonodos/patologia , Subpopulações de Linfócitos/patologia , Macrófagos/patologia , Masculino , Metaloproteinase 9 da Matriz/biossíntese , Metaloproteinase 9 da Matriz/genética , Neurite Autoimune Experimental/enzimologia , Neurite Autoimune Experimental/patologia , Óxido Nítrico Sintase Tipo II/biossíntese , Óxido Nítrico Sintase Tipo II/genética , Piridinas/farmacologia , RNA Mensageiro/biossíntese , RNA Mensageiro/genética , Ratos , Ratos Endogâmicos Lew , Nervo Isquiático/metabolismo , Nervo Isquiático/patologia
8.
Chin J Integr Med ; 13(1): 37-40, 2007 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-17578316

RESUMO

OBJECTIVE: To study the effect of Shenqi Fuzheng Injection (SFI) on cellular immune in patients with mammary cancer (MC) after chemotherapy. METHODS: One hundred and ten patients with MC were randomly assigned to two groups. The 58 patients in the tested group were treated with SFI in cooperation with chemotherapy of CAF protocol (Cyclophosphamide, Doxorubicin and Fluorouracil), while the 52 patients in the control group were treated with chemotherapy of the same protocol alone. Changes of the patients' quality of life (QOF), adverse reaction that occurred, peripheral lymphocyte count and killing activity of single karyocyte before and after treatment between the two groups were compared. RESULTS: Patients' QOF elevating rate after treatment in the tested group and the control group was 34.5% and 13.5% respectively; The lowering of peripheral blood cell count of WBC, platelet and lymphocyte as well as that of the killing activity of single peripheral karyocyte on various kinds of MC cells were all milder and recovery sooner than those in the control group. CONCLUSION: SFI in combination with chemotherapy in treating MC could reduce the occurrence of adverse reaction to chemotherapy, improve clinical symptoms, elevate QOF and enhance immunity in patients with MC.


Assuntos
Protocolos de Quimioterapia Combinada Antineoplásica/uso terapêutico , Neoplasias da Mama/terapia , Carcinoma Ductal de Mama/imunologia , Carcinoma Ductal de Mama/terapia , Medicamentos de Ervas Chinesas/administração & dosagem , Imunidade Celular/efeitos dos fármacos , Adulto , Idoso , Contagem de Células Sanguíneas , Neoplasias da Mama/sangue , Neoplasias da Mama/fisiopatologia , Carcinoma Ductal de Mama/sangue , Carcinoma Ductal de Mama/fisiopatologia , Morte Celular , Terapias Complementares , Ciclofosfamida/uso terapêutico , Doxorrubicina/uso terapêutico , Medicamentos de Ervas Chinesas/efeitos adversos , Medicamentos de Ervas Chinesas/uso terapêutico , Feminino , Fluoruracila/uso terapêutico , Humanos , Injeções , Subpopulações de Linfócitos/patologia , Pessoa de Meia-Idade , Monócitos/imunologia , Qualidade de Vida
9.
Clin Exp Rheumatol ; 21(1): 57-62, 2003.
Artigo em Inglês | MEDLINE | ID: mdl-12673890

RESUMO

OBJECTIVE: Crude preparations of Fun-boi (Stephania tetrandra), a traditional antirheumatic herb, have been reported to have immunomodulatory effects on both cell-mediated and humoral immunity in vitro, but little is known about the mode of action in vivo. The objective of this study was therefore to evaluate the efficacy of Fun-boi against arthritis and its effect on the immune system. METHODS: Mice were divided into the following 3 groups of 7 mice each: 1) a normal group, not treated to cause collagen-induced arthritis (CIA), received water orally; 2) a control group with CIA received water orally; and 3) the Fun-boi group with CIA, received Fun-boi (3 mg/g body weight/day) orally. We analyzed the arthritis score, the serum anti-type II collagen (CII) antibody level, and the percentage of the following lymphocyte subsets from lymphoid organs: B220, CD3/CD4, CD3/CD8 and CD40L/CD4 lymphocytes from blood or lymph nodes; and CD4-CD8-, CD4+CD8+, CD4+CD8- and CD4-CD8+ from the thymus. RESULTS: Fun-boi therapy markedly reduced the severity of arthritis (p < 0.001) and tended to reduce the serum anti-CII antibody level (p = 0.06). Whereas CII immunization of DBA/1J mice caused a significant redistribution of CD3/CD8 lymphocytes from blood or lymph nodes, Fun-boi therapy caused significant normalization of the same types of lymphocyte subsets from lymph nodes, but did not affect the CD4 or CD4/CD40L lymphocyte subsets. CONCLUSION: These results demonstrate that Fun-boi therapy exerts therapeutic effects in CIA mice, possibly by causing immunomodulatory effects at specific sites.


Assuntos
Adjuvantes Imunológicos/uso terapêutico , Artrite Experimental/tratamento farmacológico , Medicamentos de Ervas Chinesas/uso terapêutico , Fitoterapia , Stephania tetrandra , Adjuvantes Imunológicos/administração & dosagem , Administração Oral , Animais , Artrite Experimental/fisiopatologia , Colágeno Tipo II/imunologia , Modelos Animais de Doenças , Medicamentos de Ervas Chinesas/administração & dosagem , Linfonodos/efeitos dos fármacos , Linfonodos/patologia , Subpopulações de Linfócitos/efeitos dos fármacos , Subpopulações de Linfócitos/patologia , Masculino , Camundongos , Camundongos Endogâmicos DBA , Raízes de Plantas/química , Plantas Medicinais/química , Índice de Gravidade de Doença , Resultado do Tratamento
10.
J Rheumatol ; 29(8): 1601-8, 2002 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-12180716

RESUMO

OBJECTIVE: To investigate the effect of hochu-ekki-to (HET) decoction on the development of collagen-induced arthritis (CIA) in mice. METHODS: CIA was induced in male DBA/IJ mice by immunization with 2 injections of bovine type II collagen (CII). HET was orally administered at different doses and with different schedules. The incidence of arthritis, arthritis index, levels of anti-CII antibody, interleukin 6 (IL-6), and tumor necrosis factor-alpha (TNF-alpha) and lymphocyte subsets were examined. RESULTS: HET caused suppression of CIA development in a dose dependent fashion and exerted a suppressive effect on CIA when administered from the first CII immunization or from the onset of CIA, but not when administered for 2 weeks before CII immunization. HET inhibited the production of specific anti-CII antibody, IL-6, and TNF-alpha, and tended to normalize the proportions of cells in lymphocyte subsets. CONCLUSION: HET suppresses the development of CIA, and HET redistributes the population of lymphocytes in lymph node and blood and inhibits IL-6 and TNF-alpha secretion in CIA mice.


Assuntos
Antirreumáticos/uso terapêutico , Artrite Experimental/prevenção & controle , Medicamentos de Ervas Chinesas/uso terapêutico , Adjuvantes Imunológicos/administração & dosagem , Animais , Antirreumáticos/administração & dosagem , Antirreumáticos/química , Artrite Experimental/sangue , Artrite Experimental/patologia , Bovinos , Cromatografia Líquida de Alta Pressão , Colágeno Tipo II/administração & dosagem , Colágeno Tipo II/imunologia , Modelos Animais de Doenças , Relação Dose-Resposta a Droga , Esquema de Medicação , Medicamentos de Ervas Chinesas/administração & dosagem , Medicamentos de Ervas Chinesas/química , Interleucina-6/sangue , Articulações/efeitos dos fármacos , Articulações/patologia , Subpopulações de Linfócitos/efeitos dos fármacos , Subpopulações de Linfócitos/patologia , Masculino , Camundongos , Camundongos Endogâmicos DBA , Índice de Gravidade de Doença , Fatores de Tempo , Fator de Necrose Tumoral alfa/análise
11.
J Nutr ; 132(7): 2019-27, 2002 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-12097686

RESUMO

Excessive intake of saturated fatty acids and/or linoleic acid favors the induction of an array of lipid mediators and cytokines enhancing inflammatory responses. Conversely, dietary supplementation with (n-3) fatty acids or vitamin D ameliorates inflammation and autoimmune diseases. Although it was well accepted that conjugated linoleic acid (CLA) prevented diseases with a common inflammatory pathogenesis (i.e., cancer and atherosclerosis), no studies were available on the roles of CLA in mucosal inflammation. The present study was designed to investigate the anti-inflammatory actions and molecular mechanisms underlying the regulation of colonic health by CLA. We hypothesized that colonic inflammation can be ameliorated by dietary CLA supplementation. To test this hypothesis, inflammation of the colonic mucosa was triggered by challenging pigs fed either soybean oil-supplemented or CLA-supplemented diets with an enteric bacterial pathogen (i.e., Brachyspira hyodysenteriae). Immunoregulatory cytokines and peroxisome proliferator-activated receptor-gamma (PPAR-gamma) mRNA expression were assayed in colonic lymph nodes and colon of pigs. Colonic mucosal lesions and lymphocyte subset distribution were evaluated by histology and immunohistochemistry. Supplementation of CLA in the diet before the induction of colitis decreased mucosal damage; maintained cytokine profiles (i.e., interferon-gamma and interleukin-10) and lymphocyte subset distributions (i.e., CD4+ and CD8+), resembling those of noninfected pigs; enhanced colonic expression of PPAR-gamma; and attenuated growth failure. Therefore, CLA fed preventively before the onset of enteric disease attenuated inflammatory lesion development and growth failure.


Assuntos
Fenômenos Fisiológicos da Nutrição Animal , Colite/microbiologia , Colite/prevenção & controle , Ácido Linoleico/farmacologia , Infecções por Spirochaetales , Animais , Colite/patologia , Colo/metabolismo , Colo/patologia , Citocinas/genética , Dieta , Gorduras na Dieta/administração & dosagem , Ácidos Graxos/administração & dosagem , Transtornos do Crescimento/etiologia , Mucosa Intestinal/patologia , Ácido Linoleico/administração & dosagem , Subpopulações de Linfócitos/patologia , RNA Mensageiro/metabolismo , Receptores Citoplasmáticos e Nucleares/genética , Infecções por Spirochaetales/complicações , Suínos , Doenças dos Suínos , Fatores de Transcrição/genética
12.
J Cardiothorac Vasc Anesth ; 8(5): 532-5, 1994 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-7803741

RESUMO

Lymphocytes and their subset counts were determined in 30 cardiac surgery patients during cardiopulmonary bypass (CPB) with or without use of an autotransfusion device. In the autotransfusion group, centrifuged and washed autologous red blood cells (median 400 mL [range 200-770 mL]) and in the control group corresponding amounts of homologous packed red blood cells (median 500 mL [range 250-750 mL]) were transfused after declamping the aorta. The percentages of T lymphocytes (CD3) and T cytotoxic cells (CD8) increased in both groups (CD3 up to 5%, P < 0.05 and CD8 up to 35%, P < 0.01), but the percentage of T helper cells (CD4) did not change. The ratio of CD4/CD8 cells decreased (up to 34%, P < 0.01). The percentage of naive resting T cells (CD45RA) increased slightly (up to 8%, P < 0.05) whereas the percentages of memory T cells (CD45RO), T cells with IL-2 receptor (CD25), and natural killer cells (CD16) remained unaltered. The percentage of HLA-DR positive lymphocytes increased during CPB (up to 18%, P < 0.05), but it was decreased thereafter (up to 16%, P < 0.05). The percentage of monocytes (CD14) decreased first during CPB in both groups (up to 32%, P < 0.01), but it was higher in the autotransfusion device group (decreased 29% from initial value) than in the control group (decreased 65% from initial value) at the end of CPB (P < 0.05). This study shows that extracorporeal circulation has an effect on lymphocytes and their subset counts. The changes were slightly immunosuppressive. By contrast, use of autotransfusion devices had only minor effects.


Assuntos
Transfusão de Sangue Autóloga/instrumentação , Ponte Cardiopulmonar , Contagem de Linfócitos , Subpopulações de Linfócitos/patologia , Linfócitos/patologia , Linfócitos T/patologia , Transfusão de Sangue , Relação CD4-CD8 , Linfócitos T CD4-Positivos/patologia , Linfócitos T CD8-Positivos/patologia , Feminino , Antígenos HLA-DR/análise , Humanos , Memória Imunológica , Células Matadoras Naturais/patologia , Contagem de Leucócitos , Ativação Linfocitária , Masculino , Pessoa de Meia-Idade , Monócitos/patologia , Receptores de Interleucina-2/análise , Linfócitos T Citotóxicos/patologia , Linfócitos T Auxiliares-Indutores/patologia
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