RESUMO
The leaves of the tree Opilia celtidifolia have a long tradition for being used in Mali and other West African countries against various ailments such as for wound healing. Previous studies on polysaccharides from these leaves showed the presence of pectic-like polymers with an effect on the human complement system as well as the ability to activate macrophages. The present study shows that bioactive arabinogalactans isolated by water of 50°C could be separated into two acidic fractions, Oc50A1 and Oc50A2. The former could, by gel filtration on Sephacryl S-400, be separated into two fractions, which were further purified on a Superdex 200 column to give the fractions Oc50A1.I.pur and Oc50A1.II.pur. These fractions were subjected to chemical and biological studies. The polysaccharides consisted mainly of heavily branched type II arabinogalactans and minor amounts of rhamnogalacturonan I regions. The isolated polymers had a high human complement-fixating ability, as well as the ability to stimulate rat macrophages and dendritic cells (DCs) and to induce B cell proliferation. These effects were especially pronounced for the higher molecular weight fraction of Oc50A1.I.pur. The fractions Oc50A1.I.pur and Oc501.II.pur stimulated secretion of pro-inflammatory cytokines from purified B cells or DCs. Collectively, these results indicate that the arabinogalactan type II polymers present in the leaves of O. celtidifolia may be used to develop medical devices for regulating inflammatory processes.
Assuntos
Galactanos/química , Galactanos/farmacologia , Extratos Vegetais/química , Extratos Vegetais/farmacologia , Folhas de Planta/química , Árvores/química , Animais , Linfócitos B/citologia , Linfócitos B/metabolismo , Proliferação de Células/efeitos dos fármacos , Ativação do Complemento/efeitos dos fármacos , Testes de Fixação de Complemento/métodos , Proteínas do Sistema Complemento/química , Proteínas do Sistema Complemento/metabolismo , Células Dendríticas/citologia , Células Dendríticas/metabolismo , Galactanos/isolamento & purificação , Humanos , Inflamação/tratamento farmacológico , Inflamação/metabolismo , Ativação de Macrófagos/efeitos dos fármacos , Macrófagos/citologia , Macrófagos/metabolismo , Mali , Extratos Vegetais/isolamento & purificação , Ratos , Cicatrização/efeitos dos fármacosAssuntos
Humanos , Técnicas de Laboratório Clínico , Técnicas Imunológicas , Testes Imunológicos/métodos , Linfócitos B/imunologia , Biópsia , Células Produtoras de Anticorpos/imunologia , Doenças Transmissíveis/imunologia , Formação de Anticorpos/imunologia , Imunidade Celular/imunologia , Imunoeletroforese , Sinais em Homeopatia , Linfócitos T/imunologia , Testes de Fixação de Complemento/métodosRESUMO
The present paper describes the optimisation of a liquid phase [125I] C1q binding assay for the detection of circulating immune complexes. Blood must be collected without any anticoagulant. The serum can be stored or transported as usual without damage. A 90 min incubation is needed to reach the equilibrium of the reaction; polyethylene glycol (2,5% final concentration) is added to precipitate the [125I] C1q-CIC complex. By its simplicity and reproducibility, this test can be currently employed, especially in the case of treatment by plasmapheresis.