RESUMO
OBJECTIVES: Contamination of surface waters is a major health threat for all living creatures. Some types of blue-green algae that naturally occur in fresh water, are able to produce various toxins, like Microcystins (MCs). Microcystin-leucine arginine (MC-LR) produced by Microcystis aeruginosa is the most toxic and abundant isoforms of MCs, and it causes hepatotoxicity. The present article reviews preclinical experiments examined different treatments, including herbal derivatives, dietary supplements and drugs against MC-LR hepatotoxicity. METHODS: We searched scientific databases Web of Science, Embase, Medline (PubMed), Scopus, and Google Scholar using relevant keywords to find suitable studies until November 2023. RESULTS: MC-LR through Organic anion transporting polypeptide superfamily transporters (OATPs) penetrates and accumulates in hepatocytes, and it inhibits protein phosphatases (PP1 and PP2A). Consequently, MC-LR disturbs many signaling pathways and induces oxidative stress thus damages cellular macromolecules. Some protective agents, especially plants rich in flavonoids, and natural supplements, as well as chemoprotectants were shown to diminish MC-LR hepatotoxicity. CONCLUSION: The reviewed agents through blocking the OATP transporters (nontoxic nostocyclopeptide-M1, captopril, and naringin), then inhibition of MC-LR uptake (naringin, rifampin, cyclosporin-A, silymarin and captopril), and finally at restoration of PPAse activity (silybin, quercetin, morin, naringin, rifampin, captopril, azo dyes) exert hepatoprotective effect against MC-LR.
Assuntos
Doença Hepática Induzida por Substâncias e Drogas , Microcistinas , Microcistinas/toxicidade , Humanos , Doença Hepática Induzida por Substâncias e Drogas/tratamento farmacológico , Toxinas Marinhas/toxicidade , Animais , Fígado/efeitos dos fármacos , Fígado/metabolismo , Suplementos Nutricionais , Substâncias Protetoras/farmacologia , Substâncias Protetoras/uso terapêuticoRESUMO
A variety of studies have investigated the toxic effects of microplastics (MPs) on microalgae, but few of them considered their influence on dinoflagellate toxins production, which could cause significant ecological safety concerns in coastal areas. This research investigated the impacts of 5 µg L-1 and 5 mg L-1 polystyrene (PS) MPs on the changes of paralytic shellfish toxins (PSTs) production and their relationship with cellular oxidative stress of Alexandrium tamarense, a common harmful algal blooms causative dinoflagellate. The results showed elevation of reactive oxygen species (ROS) levels, activation of antioxidant system and overproduction of PSTs were positively correlated under PS MPs exposure (especially under 5 mg L-1 PS MPs), and the PSTs changes were eliminated by the ROS inhibitor. Further transcriptomic analysis revealed that ROS could enhance biosynthesis of glutamate, providing raw materials for PSTs precursor arginine, accompanied with enhanced acetyl-CoA and ATP production, finally leading to the overproduction of PSTs. Moreover, the oxidative intracellular environments might block the reduction process from STX to C1&C2, leading to the increase of STX and decrease of C1&C2 proportions. This work brings the first evidence that ROS could mediate PSTs production and compositions of Alexandrium under MPs exposure, with important scientific and ecological significance.
Assuntos
Dinoflagellida , Plásticos , Plásticos/farmacologia , Espécies Reativas de Oxigênio , Microplásticos/toxicidade , Toxinas Marinhas/toxicidade , Frutos do MarRESUMO
The Galician Rías (northwestern Spain) are periodically affected by harmful algal blooms (HABs), mostly dinoflagellates, which pose a challenge to aquaculture activities due to the accumulation of biotoxins in shellfish. Typically, reddish blooms in the Rías are associated with non-toxic species like Noctiluca scintillans, with a few exceptions such as Alexandrium minutum, a producer of paralytic shellfish toxins (PST). Here, a useful approach is presented for monitoring reddish blooms through satellite imagery based on three case studies, two of them belonged to monospecific blooms of red Noctiluca scintillans, and the third to a bloom of Alexandrium spp. dominated by A. tamarense. In every case, a propulsive index was evaluated using Sentinel-2A/B satellites, which provide high spatial and spectral resolutions, combined with adequate atmospheric and sunglint correction by using the ACOLITE and C2RCC processors. This approach offers a simple and feasible method to accurately and timely map blooms of red N. scintillans and Alexandrium spp. in the study area, useful to detect the distribution of reddish blooms with synoptic observations for monitoring and aquaculture management purposes. Conversely, Sentinel-3A/B satellites with a relatively coarser spatial resolution, lacking adequate visualization and mapping of the extent of small blooms, did not accurately detect bloom footprints in the coastal bay region, although this sensor displays a set of suitable multispectral bands.
Assuntos
Dinoflagellida , Proliferação Nociva de Algas , Toxinas Marinhas/toxicidade , Espanha , AquiculturaRESUMO
Seafood represents a significant part of the human staple diet. In the recent years, the identification of emerging lipophilic marine toxins has increased, leading to the potential for consumers to be intoxicated by these toxins. In the present work, we investigate the presence of lipophilic marine toxins (both regulated and emerging) in commercial seafood products from non-European locations, including mussels Mytilus chilensis from Chile, clams Tawerea gayi and Metetrix lyrate from the Southeast Pacific and Vietnam, and food supplements based on mussels formulations of Perna canaliculus from New Zealand. All these products were purchased from European Union markets and they were analyzed by UPLC-MS/MS. Results showed the presence of the emerging pinnatoxin-G in mussels Mytilus chilensis at levels up to 5.2 µg/kg and azaspiracid-2 and pectenotoxin-2 in clams Tawera gayi up to 4.33 µg/kg and 10.88 µg/kg, respectively. This study confirms the presence of pinnatoxins in Chile, one of the major mussel producers worldwide. Chromatograms showed the presence of 13-desmethyl spirolide C in dietary supplements in the range of 33.2-97.9 µg/kg after an extraction with water and methanol from 0.39 g of the green lipped mussels powder. As far as we know, this constitutes the first time that an emerging cyclic imine toxin in dietary supplements is reported. Identifying new matrix, locations, and understanding emerging toxin distribution area are important for preventing the risks of spreading and contamination linked to these compounds.
Assuntos
Ração Animal/análise , Suplementos Nutricionais/análise , Iminas/análise , Toxinas Marinhas/análise , Mytilus/química , Perna (Organismo)/química , Alimentos Marinhos/análise , Compostos de Espiro/análise , Ração Animal/toxicidade , Animais , Aquicultura , Suplementos Nutricionais/toxicidade , Contaminação de Alimentos , Iminas/toxicidade , Toxinas Marinhas/toxicidade , Medição de Risco , Compostos de Espiro/toxicidadeRESUMO
Food supplements with microalgae are becoming increasingly abundant and can be easily found anywhere. The most popular products are based on cyanophytes, such as Aphanizomenon flos-aquae, Arthrospira platensis and Limnospira maxima, or on chlorophytes, such as Chlorella or Haematoccus. Although they are all advertised as being very beneficial for health, these products might be harmful because they may contain cyanotoxins and other contaminants, and no information on production methods or strain origins is usually provided. While legislation on the presence of microcystins in waters for different uses is clear, toxicological analyses are not compulsory for food supplements, nor for analyzing anatoxins. Given the potential risk of eating contaminated food, cyanotoxins, heavy metals and the presence of other contaminant organisms were analyzed in 10 microalgae food supplements. Microcystin-LR and anatoxin-a were detected in three analyzed products, and in both cyanophyte- and chlorophyte-based products. The light microscope study revealed the presence of different potentially harmful microbial contaminants. The ICP (OES) analyses detected high concentrations of some heavy metals, especially Pb. The results emphasize the need to promote the better control of food products containing microalgae, and to develop standard methodologies to analyze cyanotoxins and potential toxic compounds to protect consumer health.
Assuntos
Suplementos Nutricionais/análise , Contaminação de Alimentos , Toxinas Marinhas/análise , Microalgas/química , Microcistinas/análise , Tropanos/análise , Cromatografia Líquida de Alta Pressão , Qualidade de Produtos para o Consumidor , Toxinas de Cianobactérias , Suplementos Nutricionais/toxicidade , Toxinas Marinhas/toxicidade , Microcistinas/toxicidade , Medição de Risco , Espectrometria de Massas por Ionização por Electrospray , Tropanos/toxicidadeRESUMO
This study evaluated the effect of dietary inclusion of lyophilized açaí Euterpe oleracea (LEO) on redox status of shrimp Litopenaeus vannamei (initial weight 1.5 ± 0.39 g) upon exposure to cyanotoxin nodularin (NOD) in bioflocs system. Three hundred juvenile shrimps were randomly divided into two groups and fed twice a day with two diets: one containing 0.00 (control diet) and the other 10.0% LEO (w/w) for 30-days. After the feeding period, both shrimp groups were submitted to three treatments (14 L; 7 shrimp/tank) with different concentrations of cyanotoxin NOD (0.00; 0.25; and 1.00 µg/L) dissolved in water with 96 h of exposure. Then, the shrimps were sampled (n = 15/treatment) for the determination of reduced glutathione (GSH), the activity of glutathione-S-transferase (GST), sulfhydryl groups associated to proteins (P-SH), and lipid peroxidation (TBARS) in the hepatopancreas, gills and muscle. The NOD accumulation was measured in the muscle. The results revealed that dietary LEO significantly increased GSH levels in the hepatopancreas and gills of the shrimps exposed to NOD. Toxin exposure did not modify GST activity in all organs. Muscle TBARS levels were lower in the shrimp fed with the LEO diet and exposed to NOD. The NOD toxin did not accumulate in the muscle but notably was detected in the control groups fed or not with dietary LEO. Açaí was able to induce the antioxidant system of L. vannamei, as well as lowered the oxidative damage in shrimps exposed to NOD, suggesting its use as a chemoprotectant against cyanotoxins.
Assuntos
Toxinas Bacterianas/toxicidade , Suplementos Nutricionais/análise , Euterpe/química , Toxinas Marinhas/toxicidade , Penaeidae/imunologia , Peptídeos Cíclicos/toxicidade , Substâncias Protetoras/farmacologia , Ração Animal/análise , Animais , Dieta/veterinária , Liofilização , Nodularia , Oxirredução , Distribuição AleatóriaRESUMO
Domoic acid (DA) is a naturally occurring neurotoxin known to harm marine animals. DA-producing algal blooms are increasing in size and frequency. Although chronic exposure is known to produce brain lesions, the influence of DA toxicosis on behavior in wild animals is unknown. We showed, in a large sample of wild sea lions, that spatial memory deficits are predicted by the extent of right dorsal hippocampal lesions related to natural exposure to DA and that exposure also disrupts hippocampal-thalamic brain networks. Because sea lions are dynamic foragers that rely on flexible navigation, impaired spatial memory may affect survival in the wild.
Assuntos
Hipocampo/efeitos dos fármacos , Ácido Caínico/análogos & derivados , Toxinas Marinhas/toxicidade , Neurotoxinas/toxicidade , Leões-Marinhos/fisiologia , Memória Espacial/efeitos dos fármacos , Animais , Eutrofização , Hipocampo/fisiologia , Ácido Caínico/metabolismo , Ácido Caínico/toxicidade , Neurotoxinas/metabolismo , Tálamo/efeitos dos fármacos , Tálamo/fisiologiaRESUMO
A multi-method approach was employed to compare the responses of Glutatione Transferases (GSTs) in the gills and hepatopancreas of Venerupis philippinarum to microcystins (MCs) toxicity. In this way, using the cytosolic fraction, the enzymatic activity of GSTs, superoxide dismutase (SOD), serine/threonine protein phosphatases (PPP2) along with the gene expression levels of four GST isoforms (pi, mu, sigma1, sigma2) were investigated in both organs of the clams exposed for 24 h to 10, 50 and 100 µg L(-1) of MC-LR. Cytosolic GSTs (cGSTs) from both organs of the high dose exposed clams were purified by glutathione-agarose affinity chromatography, characterized kinetically and the changes in the expression of cGSTs of the gills identified using a proteomic approach. MC-LR caused an increase in GST enzyme activity, involved in conjugation reactions, in both gills and hepatopancreas (100 µg L(-1) exposure). SOD activity, an indicator of oxidative stress, showed signiï¬cantly elevated levels in the hepatopancreas only (50 and 100 µg L(-1) exposure). No significant changes were found in PPP2 activity, the main target of MCs, for both organs. Transcription responses revealed an up-regulation of sigma2 in the hepatopancreas at the high dose, but no significant changes were detected in the gills. Kinetic analysis evidenced differences between gills of exposed and non-exposed extracts. Using proteomics, qualitative and quantitative differences were found between the basal and inducible cGSTs. Overall, results suggest a distinct role of GST system in counteracting MCs toxicity between the gills and the hepatopancreas of V. philippinarum, revealing different roles between GST isoforms within and among both organs.
Assuntos
Toxinas Bacterianas/toxicidade , Brânquias/efeitos dos fármacos , Glutationa Transferase/genética , Glutationa Transferase/metabolismo , Hepatopâncreas/efeitos dos fármacos , Toxinas Marinhas/toxicidade , Microcistinas/toxicidade , Animais , Bivalves , Regulação Enzimológica da Expressão Gênica/efeitos dos fármacos , Brânquias/enzimologia , Hepatopâncreas/enzimologia , Isoenzimas/genética , Isoenzimas/metabolismo , Proteína Fosfatase 2/metabolismo , Superóxido Dismutase/metabolismoRESUMO
Several species of crabs are resistant to paralytic shellfish toxins (PSTs) and/or pufferfish toxin, tetrodotoxin, regardless of toxification by the toxins. The shore crab Thalamita crenata, which inhabits Leizhou Peninsula, China, is tolerant to PST toxicity, and the hemolymph has neutralizing effects against the lethal activity of PST. In the present study, we investigated the PST neutralizing factors in the hemolymph from T. crenata and successfully separated PST-binding proteins by PST-ligand affinity chromatography. The neutralization factors, obtained in the fraction with a molecular weight over 10 kDa by ultrafiltration, were susceptible to proteases such as alcalase, animal complex proteases, pancreatin, and papain. The PST-binding protein had high dose-dependent neutralization effects on PST toxicity. The PST-binding activity of the protein was stable at 25 °C and then decreased with an increase in temperature; heating at 65 °C for 60 min eliminated the initial activity by two-thirds. The PST-binding activity was strongly inhibited in the presence of Mg(2+) and Ca(2+), but not Na(+) and K(+). The PST-binding capability of the protein differed among PST components in descending order of neosaxitoxin, gonyautoxins 1 and 4, saxitoxin, and gonyautoxins 2 and 3, suggesting a structure-activity relationship in PST binding.
Assuntos
Antídotos/uso terapêutico , Proteínas de Artrópodes/uso terapêutico , Braquiúros/química , Hemolinfa/química , Toxinas Marinhas/antagonistas & inibidores , Intoxicação por Frutos do Mar/tratamento farmacológico , Animais , Antídotos/química , Antídotos/isolamento & purificação , Antídotos/metabolismo , Proteínas de Artrópodes/química , Proteínas de Artrópodes/isolamento & purificação , Proteínas de Artrópodes/metabolismo , Bioensaio , Braquiúros/crescimento & desenvolvimento , China , Cromatografia de Afinidade , Estabilidade de Medicamentos , Temperatura Alta/efeitos adversos , Ligantes , Masculino , Toxinas Marinhas/química , Toxinas Marinhas/toxicidade , Camundongos , Peso Molecular , Oceano Pacífico , Estabilidade Proteica , Proteólise , Saxitoxina/análogos & derivados , Saxitoxina/antagonistas & inibidores , Saxitoxina/química , Saxitoxina/toxicidade , Intoxicação por Frutos do Mar/etiologiaRESUMO
Shark cartilage products are marketed as dietary supplements with claimed health benefits for animal and human use. Shark fin and cartilage products sold as extracts, dry powders and in capsules are marketed based on traditional Chinese medicine claims that it nourishes the blood, enhances appetite, and energizes multiple internal organs. Shark cartilage contains a mixture of chondroitin and glucosamine, a popular nutritional supplement ingested to improve cartilage function. Sharks are long-lived apex predators, that bioaccumulate environmental marine toxins and methylmercury from dietary exposures. We recently reported detection of the cyanobacterial toxin ß-N-methylamino-l-alanine (BMAA) in the fins of seven different species of sharks from South Florida coastal waters. Since BMAA has been linked to degenerative brain diseases, the consumption of shark products may pose a human risk for BMAA exposures. In this report, we tested sixteen commercial shark cartilage supplements for BMAA by high performance liquid chromatography (HPLC-FD) with fluorescence detection and ultra performance liquid chromatography/mass spectrometry/mass spectrometry (UPLC-MS/MS). Total mercury (Hg) levels were measured in the same shark cartilage products by cold vapor atomic fluorescence spectrometry (CVAFS). We report here that BMAA was detected in fifteen out of sixteen products with concentrations ranging from 86 to 265µg/g (dry weight). All of the shark fin products contained low concentrations of Hg. While Hg contamination is a known risk, the results of the present study demonstrate that shark cartilage products also may contain the neurotoxin BMAA. Although the neurotoxic potential of dietary exposure to BMAA is currently unknown, the results demonstrate that shark cartilage products may contain two environmental neurotoxins that have synergistic toxicities.
Assuntos
Diamino Aminoácidos/toxicidade , Suplementos Nutricionais , Contaminação de Medicamentos , Mercúrio/toxicidade , Neurotoxinas/toxicidade , Extratos de Tecidos/toxicidade , Diamino Aminoácidos/análise , Toxinas Bacterianas/toxicidade , Cromatografia Líquida de Alta Pressão , Cromatografia Líquida , Cianobactérias/química , Toxinas de Cianobactérias , Meio Ambiente , Toxinas Marinhas/toxicidade , Mercúrio/análise , Microcistinas/toxicidade , Neurotoxinas/análise , Espectrometria de Massas em Tandem , Extratos de Tecidos/químicaRESUMO
Microcystins (MCs) are a family of cyclic heptapeptides that are produced by blooming algae Microcystis. MCs have been implicated in the development of liver cancer, necrosis and even intrahepatic bleeding. Effective prophylactic approaches and complete removal of MCs are urgently needed. Accumulating evidence suggests that microcystin-LR (MC-LR)-induced damage is accompanied by oxidative stress. Supplementation of Se can enhance resistance to oxidative stress. Therefore, in the present study, we investigated the protective effects of κ-Selenocarrageenan (Se-Car), a kind of organic Se compound, in Balb/c mice exposed to MC-LR. Our results proved that Se-Car could significantly ameliorate the hepatic damage induced by MC-LR, including serum markers of liver dysfunction, oxidative damages and histological alterations. Furthermore, Se-Car could significantly alleviate the up-regulation of the molecular targets indicating mitochondrial dysfunction and endoplasmic reticulum stress induced by MC-LR. In conclusion, Se-Car showed clear protection against toxicity induced by MC-LR. Thus, Se-Car could be useful as a new category of anti-MC-LR toxicity reagent.
Assuntos
Antitoxinas/uso terapêutico , Toxinas Bacterianas/antagonistas & inibidores , Carragenina/uso terapêutico , Insuficiência Hepática/prevenção & controle , Fígado/efeitos dos fármacos , Toxinas Marinhas/antagonistas & inibidores , Microcistinas/antagonistas & inibidores , Compostos Organosselênicos/uso terapêutico , Proteínas Adaptadoras de Transdução de Sinal , Animais , Toxinas Bacterianas/toxicidade , Biomarcadores/sangue , Proteínas de Transporte/agonistas , Proteínas de Transporte/antagonistas & inibidores , Proteínas de Transporte/genética , Proteínas de Transporte/metabolismo , Proteínas de Ciclo Celular , Estresse do Retículo Endoplasmático/efeitos dos fármacos , Fatores de Iniciação em Eucariotos , Insuficiência Hepática/induzido quimicamente , Insuficiência Hepática/metabolismo , Insuficiência Hepática/patologia , Peroxidação de Lipídeos/efeitos dos fármacos , Fígado/metabolismo , Fígado/patologia , Fígado/fisiopatologia , Masculino , Toxinas Marinhas/toxicidade , Camundongos , Camundongos Endogâmicos BALB C , Microcistinas/toxicidade , Microcystis/metabolismo , Mitocôndrias Hepáticas/efeitos dos fármacos , Mitocôndrias Hepáticas/metabolismo , Mitocôndrias Hepáticas/patologia , Estresse Oxidativo/efeitos dos fármacos , Fosfoproteínas/agonistas , Fosfoproteínas/antagonistas & inibidores , Fosfoproteínas/genética , Fosfoproteínas/metabolismo , Distribuição Aleatória , Transdução de Sinais/efeitos dos fármacos , Análise de SobrevidaRESUMO
Aphanizomenon flos-aquae (A. flos-aquae) is a source of neurotoxins known as aphantoxins or paralytic shellfish poisons (PSPs) that present a major threat to the environment and to human health. Generally, altered neurological function is reflected in behavior. Although the molecular mechanism of action of PSPs is well known, its neurobehavioral effects on adult zebrafish and its relationship with altered neurological functions are poorly understood. Aphantoxins purified from a natural isolate of A. flos-aquae DC-1 were analyzed by HPLC. The major analogs found in the toxins were the gonyautoxins 1 and 5 (GTX1 and GTX5; 34.04% and 21.28%, respectively) and the neosaxitoxin (neoSTX, 12.77%). Zebrafish (Danio rerio) were intraperitoneally injected with 5.3 and 7.61 µg STXeq/kg (low and high dose, respectively) of A. flos-aquae DC-1 aphantoxins. The swimming activity was investigated by observation combined with video at 6 timepoints from 1 to 24 h post-exposure. Both aphantoxin doses were associated with delayed touch responses, reduced head-tail locomotory abilities, inflexible turning of head, and a tailward-shifted center of gravity. The normal S-pattern (or undulating) locomotor trajectory was replaced by a mechanical motor pattern of swinging the head after wagging the tail. Finally, these fish principally distributed at the top and/or bottom water of the aquarium, and showed a clear polarized distribution pattern at 12 h post-exposure. Further analysis of neurological function demonstrated that both aphantoxin doses inhibited brain acetylcholinesterase activity. All these changes were dose- and time-dependent. These results demonstrate that aphantoxins can alter locomotor capacity, touch responses and distribution patterns by damaging the cholinergic system of zebrafish, and suggest that zebrafish locomotor behavior and acetylcholinesterase can be used as indicators for investigating aphantoxins and blooms in nature.
Assuntos
Acetilcolinesterase/metabolismo , Aphanizomenon/química , Toxinas Bacterianas/toxicidade , Encéfalo/efeitos dos fármacos , Toxinas Marinhas/toxicidade , Atividade Motora/efeitos dos fármacos , Peixe-Zebra/fisiologia , Análise de Variância , Animais , Toxinas Bacterianas/administração & dosagem , Encéfalo/enzimologia , Cromatografia Líquida de Alta Pressão , Relação Dose-Resposta a Droga , Fluorescência , Cabeça/fisiologia , Injeções Intraperitoneais , Toxinas Marinhas/administração & dosagem , Atividade Motora/fisiologia , Cauda/efeitos dos fármacos , Cauda/fisiologia , Tato/efeitos dos fármacosRESUMO
Nodularin is one of the most conspicuous and widespread pollutants that elicit water ecological hazards to fish, causing serious damage on the immune system and physiological functions. Nodularin can cause oxidative stress-induced apoptosis on fish lymphocytes. The regulatory effects of epigallocatechin-3-gallate (EGCG) at 10, 100, and 1000 µg/L levels on the antioxidant defense system and apoptosis of Carassius auratus lymphocytes exposed to a high dose of nodularin (100 µg/L) were quantified in vitro. EGCG reduced nodularin-induced oxidative damage on fish immune cells. This compound significantly increased the activities of superoxide dismutase and catalase and the level of glutathione but decreased the levels of intracellular reactive oxygen species and malondialdehyde. Flow cytometry results showed that the percentages of apoptotic cells after treatment with 10, 100, and 1000 µg/L EGCG for 12 h reached 27.9%, 19.1%, and 13.7%, respectively. By contrast, the nodularin alone-induced group showed a high percentage of apoptosis (44.2%). Western blot analysis showed the increased expression of bcl-2 and the decreased expression of bax and caspase-3 in EGCG-treated fish lymphocytes. EGCG also inhibited the potential collapse of the mitochondrial membrane. Overall, EGCG can inhibit nodularin-induced apoptosis and protect the normal immunity of fish by regulating bax/bcl-2 and blocking the downstream of mitochondrial apoptosis pathway with increased intracellular antioxidant enzyme activity.
Assuntos
Antioxidantes/metabolismo , Catequina/análogos & derivados , Carpa Dourada/metabolismo , Linfócitos/efeitos dos fármacos , Peptídeos Cíclicos/toxicidade , Poluentes Químicos da Água/toxicidade , Animais , Apoptose/efeitos dos fármacos , Western Blotting/veterinária , Catequina/metabolismo , Células Cultivadas , Relação Dose-Resposta a Droga , Feminino , Citometria de Fluxo/veterinária , Linfócitos/citologia , Linfócitos/metabolismo , Masculino , Toxinas Marinhas/toxicidade , Potencial da Membrana Mitocondrial/efeitos dos fármacos , Nodularia/química , Estresse Oxidativo/efeitos dos fármacos , Espécies Reativas de Oxigênio/metabolismoRESUMO
The acute and sub-chronic toxicities of cyanobacterial extract from Taihu Lake (PR China) on mouse (Mus musculus) were investigated in this study via intraperitoneal (i.p.) injection. Increases in liver/body weight ratios and pathological changes in mouse liver showed adverse effects at the organ level. Images from transmission electron microscopy (TEM) indicated that abnormal membrane structure occurred and that the organelles were damaged severely in the cells of liver and testis. The high dose group received i.p. injection of 12 mg lyophilized algae cells/kg body weight. Malondialdehyde (MDA) levels increased significantly in the livers of this group, along with a significant decrease in catalase (CAT) activity. These results revealed the existence of obvious oxidative stress. Comet assay results also suggested a dose-dependent relationship between DNA damage in hepatocytes/testicular cells and the amount of bloom extract administered to the mice. There was a significant increase in DNA damage compared to the control group and the genotoxicity of the cyanobacterial bloom to testicular cells was higher than in hepatocytes.
Assuntos
Toxinas Bacterianas/toxicidade , Cianobactérias/patogenicidade , Água Doce/microbiologia , Toxinas Marinhas/toxicidade , Microcistinas/toxicidade , Microbiologia da Água , Poluentes Químicos da Água/toxicidade , Animais , Toxinas Bacterianas/administração & dosagem , Peso Corporal/efeitos dos fármacos , Catalase/metabolismo , China , Toxinas de Cianobactérias , Relação Dose-Resposta a Droga , Monitoramento Ambiental , Água Doce/química , Injeções Intraperitoneais , Fígado/efeitos dos fármacos , Fígado/metabolismo , Fígado/patologia , Masculino , Malondialdeído/metabolismo , Toxinas Marinhas/administração & dosagem , Camundongos , Microcistinas/administração & dosagem , Mutagênicos/administração & dosagem , Mutagênicos/toxicidade , Superóxido Dismutase/metabolismo , Poluentes Químicos da Água/administração & dosagemRESUMO
This study evaluated the influence of toxic cyanobacterial water blooms on the blood indices of the common carp, Cyprinus carpio L. Experimental fish were exposed to a natural population of cyanobacterial water blooms (mainly Microcystis aeruginosa and M. ichthyoblabe), which contained microcystins [total concentration 133-284 µg g⻹ (DW), concentration in water 2.8-7.4 µg L⻹]. Haematological indices showed marked changes in fish exposed to the cyanobacterial population in comparison with the control group. Statistical evaluation of the influence of cyanobacterial water blooms on biochemical indices of the juvenile carp showed a distinct decrease in albumin, alanine aminotransferase, total bilirubin, calcium, cholesterol, glucose, phosphorus and iron when compared to controls. Values of red blood counts [haemoglobin, haematocrit (PCV), mean corpuscular haemoglobin and mean corpuscular haemoglobin concentration] and lactate were significantly increased compared to controls. After exposure to cyanobacterial water bloom, the carp were kept in clean water to monitor the persistence of biochemical indices. The influence of cyanobacterial populations on calcium, cholesterol, glucose, lactate, phosphorus and PCV persisted up to 28 days after conclusion of the experiment. Duration of exposure, toxicity and density of cyanobacterial water blooms had an important impact on individual haematological indices.
Assuntos
Toxinas Bacterianas/toxicidade , Carpas/sangue , Eutrofização/efeitos dos fármacos , Toxinas Marinhas/toxicidade , Microcistinas/toxicidade , Microcystis/efeitos dos fármacos , Alanina Transaminase/sangue , Animais , Toxinas Bacterianas/sangue , Bilirrubina/sangue , Glicemia/análise , Cálcio/sangue , Carpas/metabolismo , Carpas/microbiologia , Colesterol/sangue , Toxinas de Cianobactérias , Contagem de Eritrócitos/veterinária , Proteínas de Peixes/sangue , Proteínas de Peixes/metabolismo , Ferro/sangue , Ácido Láctico/sangue , Toxinas Marinhas/sangue , Microcistinas/sangue , Microcistinas/metabolismo , Fósforo/sangue , Albumina Sérica/análiseRESUMO
Besides climate change impacts on water availability and hydrological risks, the consequences on water quality is just beginning to be studied. This review aims at proposing a synthesis of the most recent existing interdisciplinary literature on the topic. After a short presentation about the role of the main factors (warming and consequences of extreme events) explaining climate change effects on water quality, the focus will be on two main points. First, the impacts on water quality of resources (rivers and lakes) modifying parameters values (physico-chemical parameters, micropollutants and biological parameters) are considered. Then, the expected impacts on drinking water production and quality of supplied water are discussed. The main conclusion which can be drawn is that a degradation trend of drinking water quality in the context of climate change leads to an increase of at risk situations related to potential health impact.
Assuntos
Água Doce/química , Efeito Estufa , Poluentes da Água/análise , Abastecimento de Água/análise , Toxinas Bacterianas/toxicidade , Carbono/análise , Cianobactérias/patogenicidade , Toxinas de Cianobactérias , Desinfetantes/análise , Secas , Monitoramento Ambiental , Inundações , Água Doce/microbiologia , Toxinas Marinhas/toxicidade , Microcistinas/toxicidade , Nitrogênio/análise , Fósforo/análise , TemperaturaAssuntos
Toxinas Bacterianas/análise , Cianobactérias/patogenicidade , Toxinas Marinhas/análise , Microcistinas/análise , Alcaloides , Animais , Toxinas Bacterianas/farmacocinética , Toxinas Bacterianas/toxicidade , Cianobactérias/isolamento & purificação , Toxinas de Cianobactérias , Suplementos Nutricionais/análise , Suplementos Nutricionais/toxicidade , Monitoramento Ambiental , Eutrofização , Peixes/metabolismo , Água Doce/análise , Água Doce/microbiologia , Sedimentos Geológicos/análise , Humanos , Toxinas Marinhas/farmacocinética , Toxinas Marinhas/toxicidade , Microcistinas/farmacocinética , Microcistinas/toxicidade , Peptídeos Cíclicos/análise , Plantas/química , Frutos do Mar/análise , Tropanos/análise , Uracila/análogos & derivados , Uracila/análiseRESUMO
Cyanobacteria are the oldest life forms on earth known to produce a broad spectrum of secondary metabolites. The functions/advantages of most of these secondary metabolites (peptides and alkaloids) are unknown, however, some of them have adverse effects in humans and wildlife, especially when ingested, inhaled or upon dermal exposure. Surprisingly, some of these cyanobacteria are ingested voluntarily. Indeed, for centuries mankind has used cyanobacteria as a protein source, primarily Spirulina species. However, recently also Aphanizomenon flos-aquae are used for the production of so called blue green algae supplements (BGAS), supposedly efficacious for treatment of various diseases and afflictions. Unfortunately, traces of neurotoxins and protein phosphatases (inhibiting compounds) have been detected in BGAS, making these health supplements a good example for human exposure to a mixture of cyanobacterial toxins in a complex matrix. The discussion of this and other possible exposure scenarios, e.g. drinking water, contact during recreational activity, or consumption of contaminated food, can provide insight into the question of whether or not our current risk assessment schemes for cyanobacterial blooms and the toxins contained therein suffice for protection of human health.
Assuntos
Toxinas Bacterianas/toxicidade , Cianobactérias/patogenicidade , Eutrofização , Toxinas Marinhas/toxicidade , Microcistinas/toxicidade , Animais , Toxinas Bacterianas/administração & dosagem , Toxinas Bacterianas/farmacocinética , Toxinas de Cianobactérias , Suplementos Nutricionais/microbiologia , Microbiologia de Alimentos , Água Doce/microbiologia , Humanos , Toxinas Marinhas/administração & dosagem , Toxinas Marinhas/farmacocinética , Microcistinas/administração & dosagem , Microcistinas/farmacocinética , Saúde Pública , Recreação , Medição de Risco , Abastecimento de ÁguaRESUMO
Four peptide toxins, SHTX I-III with crab-paralyzing activity and SHTX IV with crab lethality, were isolated from the sea anemone Stichodactyla haddoni and their primary structures elucidated by protein sequencing and cDNA cloning. SHTX I (new toxin, 28 residues), II (analogue of SHTX I, 28 residues) and III (Kunitz-type protease inhibitor, 62 residues) are potassium channel toxins and SHTX IV (48 residues) is a member of the type 2 sea anemone sodium channel toxins. The precursor protein of SHTX IV is composed of a signal peptide, propart and mature peptide, while the propart is missing in that of SHTX III. In addition to these four toxins, an epidermal growth factor-like peptide was detected in S. haddoni by RT-PCR.
Assuntos
Toxinas Marinhas/química , Toxinas Marinhas/toxicidade , Peptídeos/química , Peptídeos/toxicidade , Anêmonas-do-Mar/química , Sequência de Aminoácidos , Animais , Sequência de Bases , Clonagem Molecular , DNA Complementar/metabolismo , Toxinas Marinhas/isolamento & purificação , Dados de Sequência Molecular , Canais de Potássio/metabolismo , Alinhamento de SequênciaRESUMO
Microcystin-LR (MC-LR), involved in human and animal poisonings by cyanobacteria, has been shown to be both a potent tumour promoter in rat liver and an inhibitor of serine/threonine protein phosphatases, specifically PP1 and PP2A. The research on the genotoxic potential of MC-LR counts only few in vivo studies. In order to determine the target organs for DNA-damage induction by MC-LR, the single-cell gel electrophoresis (SCGE) or comet assay was performed in mice. Following a single oral administration of 2 and 4mg/kg bw of MC-LR, a statistically significant induction of DNA damage in blood cells was obtained after 3h. However, after an intra-peritoneal injection (ip), DNA lesions were mainly induced in the liver, but were also reported in the kidney, the intestine and the colon. The sensitivity of the ip route compared to the oral route suggested a difference in the bio-disponibility of the toxin. In any case, DNA damage was induced by MC-LR irrespective of the administration route. Among the target organs, the DNA damage induced in the intestinal tissues (ileum and colon) may contribute to an increased cancer risk.