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1.
J Gen Virol ; 99(10): 1418-1424, 2018 10.
Artigo em Inglês | MEDLINE | ID: mdl-30156527

RESUMO

Analysis of transcriptome sequence data from eggs and second-stage juveniles (J2s) of sugar beet cyst nematode (SBCN, Heterodera schachtii) identified the full-length genome of a positive-sense single-stranded RNA virus, provisionally named sugar beet cyst nematode virus 1 (SBCNV1). The SBCNV1 sequence was detected in both eggs and J2s, indicating its possible vertical transmission. The 9503-nucleotide genome sequence contains a single long open reading frame, which was predicted to encode a polyprotein with conserved domains for picornaviral structural proteins proximal to its amino terminus and RNA helicase, cysteine proteinase and RNA-dependent RNA polymerase (RdRp) conserved domains proximal to its carboxyl terminus, hallmarks of viruses belonging to the order Picornavirales. Phylogenetic analysis of the predicted SBCNV1 RdRp amino acid sequence indicated that the SBCNV1 sequence is most closely related to members of the family Secoviridae, which includes genera of nematode-transmitted plant-infecting viruses. SBCNV1 represents the first fully sequenced viral genome from SBCN.


Assuntos
Beta vulgaris/parasitologia , Picornaviridae/classificação , Picornaviridae/isolamento & purificação , Transcriptoma , Tylenchoidea/virologia , Animais , Genoma Viral , Anotação de Sequência Molecular , Fases de Leitura Aberta , Filogenia , Picornaviridae/genética , RNA Polimerase Dependente de RNA/genética , Análise de Sequência de DNA , Análise de Sequência de RNA , Homologia de Sequência de Aminoácidos , Tylenchoidea/genética , Tylenchoidea/crescimento & desenvolvimento , Proteínas Virais/genética
2.
PLoS One ; 11(1): e0147511, 2016.
Artigo em Inglês | MEDLINE | ID: mdl-26824923

RESUMO

The beet cyst nematode, Heterodera schachtii, is a major root pest that significantly impacts the yield of sugar beet, brassicas and related species. There has been limited molecular characterisation of this important plant pathogen: to identify target genes for its control the transcriptome of the pre-parasitic J2 stage of H. schachtii was sequenced using Roche GS FLX. Ninety seven percent of reads (i.e., 387,668) with an average PHRED score > 22 were assembled with CAP3 and CLC Genomics Workbench into 37,345 and 47,263 contigs, respectively. The transcripts were annotated by comparing with gene and genomic sequences of other nematodes and annotated proteins on public databases. The annotated transcripts were much more similar to sequences of Heterodera glycines than to those of Globodera pallida and root knot nematodes (Meloidogyne spp.). Analysis of these transcripts showed that a subset of 2,918 transcripts was common to free-living and plant parasitic nematodes suggesting that this subset is involved in general nematode metabolism and development. A set of 148 contigs and 183 singletons encoding putative homologues of effectors previously characterised for plant parasitic nematodes were also identified: these are known to be important for parasitism of host plants during migration through tissues or feeding from cells or are thought to be involved in evasion or modulation of host defences. In addition, the presence of sequences from a nematode virus is suggested. The sequencing and annotation of this transcriptome significantly adds to the genetic data available for H. schachtii, and identifies genes primed to undertake required roles in the critical pre-parasitic and early post-parasitic J2 stages. These data provide new information for identifying potential gene targets for future protection of susceptible crops against H. schachtii.


Assuntos
Beta vulgaris/parasitologia , Brassica/parasitologia , Proteínas de Helminto/genética , Transcriptoma , Tylenchoidea/genética , Sequência de Aminoácidos , Animais , Ontologia Genética , Sequenciamento de Nucleotídeos em Larga Escala , Interações Hospedeiro-Parasita , Estágios do Ciclo de Vida/genética , Anotação de Sequência Molecular , Dados de Sequência Molecular , Filogenia , Alinhamento de Sequência , Tylenchoidea/classificação , Tylenchoidea/crescimento & desenvolvimento , Tylenchoidea/virologia
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