RESUMO
E1 Tor Vibrio cholerae (EVC) strains may be classified into two kinds-epidemigenic (EEVC) strains and non-epidemigenic (NEEVC) strains-based on a phage-biotyping system. A large number of EEVC strains have been screened for toxigenic and putative colonization attributes. One such naturally occurring strains (designated IEM101) has been found which is devoid of genes encoding cholera toxin (CT), accessory cholera enterotoxin (ACE), zonula occludens toxin (ZOT), but possesses RS1 sequences and toxin-coregulated pilus A gene (icpA) although icpA is poorly expressed. It expresses type B pili but does not possess type C pili. It is an E1 Tor Ogawa strain and does not cause fluid accumulation in rabbit ilcal loop tests. Active immunization of rabbits with strain IEM101 elicited good protection against challenge with virulent strains of V. cholerae O1. Oral administration caused no side effects in 15 human volunteers, colonized the gut for four to ten days and elicited good immune responses.
Assuntos
Vacinas contra Cólera , Cólera/prevenção & controle , Vibrio cholerae/classificação , Animais , Tipagem de Bacteriófagos , Vacinas contra Cólera/efeitos adversos , Avaliação Pré-Clínica de Medicamentos , Humanos , Camundongos , Coelhos , Valores de Referência , Vibrio cholerae/patogenicidadeRESUMO
The presence of several active foci of infection of different etiology is an indication for complex (combined) immunization against these diseases. The scheme of complex (combined) immunization against plague, cholera and yellow fever has been experimentally substantiated and successfully tested on volunteers.
Assuntos
Vacinas contra Cólera/imunologia , Vacina contra a Peste/imunologia , Quarentena , Vacinas Virais/imunologia , Vírus da Febre Amarela/imunologia , Adolescente , Adulto , Animais , Cólera/imunologia , Cólera/prevenção & controle , Vacinas contra Cólera/efeitos adversos , Avaliação de Medicamentos , Avaliação Pré-Clínica de Medicamentos , Cobaias , Humanos , Camundongos , Camundongos Endogâmicos BALB C , Papio , Peste/imunologia , Peste/prevenção & controle , Vacina contra a Peste/efeitos adversos , Coelhos , Ratos , Vacinas Combinadas/efeitos adversos , Vacinas Combinadas/imunologia , Vacinas Virais/efeitos adversos , Febre Amarela/imunologia , Febre Amarela/prevenção & controleRESUMO
The article presents materials on experiments substantiating the method for the subcutaneous administration of chemical adsorbed typhoid vaccine by means of a jet-injector and the possibility of using this preparation in combination with cholerogen toxoid.