RESUMO
Taxonomic analysis of budding yeast strains isolated from flowers of Wisteria sinensis (Fabales, Fabaceae) abundantly visited by flying insects, mainly bees in city parks of Baku is described. The isolates forming slightly pink colonies and propagating by budding represent a hitherto unknown yeast species for which the name Starmerella caucasica is proposed. The sequences of the D1/D2 domains of the large subunit rRNA genes and the ITS1-5.8S-ITS2 regions were highly similar in the isolates and indicated a close relationship with Candida kuoi and Starmerella bombicola in the phylogenetic analysis. S. caucasica can be separated from these species by its growth on glucosamine and D-tryptophan, in vitamin-free medium and at 37°C, and its inability to grow on citrate, ethylamine, cadaverine and in media supplemented with 0.01% of cycloheximide. The type strain is 11-1071.1(T). It has been deposited in Centralbureau voor Schimmelcultures (Utrecht, the Netherlands) as CBS 12650(T), the National Collection of Agricultural and Industrial Microorganisms (Budapest, Hungary) as NCAIM Y.02030(T) and the Culture Collection of Yeasts (Bratislava, Slovakia) as CCY 90-1-1(T). The GenBank accession numbers for nucleotide sequences of S. caucasica are JX112043 (D1/D2 domain of the 26S rRNA gene) and JX112044 (ITS1-5.8S-ITS2). Mycobank: MB 800536.
Assuntos
Ascomicetos/classificação , Ascomicetos/isolamento & purificação , Wisteria/microbiologia , Ascomicetos/genética , Ascomicetos/fisiologia , Azerbaijão , Análise por Conglomerados , DNA Fúngico/química , DNA Fúngico/genética , DNA Ribossômico/química , DNA Ribossômico/genética , DNA Espaçador Ribossômico/química , DNA Espaçador Ribossômico/genética , Flores/microbiologia , Genes de RNAr , Dados de Sequência Molecular , Técnicas de Tipagem Micológica , Filogenia , Pigmentos Biológicos/metabolismo , RNA Fúngico/genética , RNA Ribossômico/genética , Análise de Sequência de DNARESUMO
This study was carried out to examine the diversity of 34 isolates collected from 11 species of leguminous trees growing in South Korea. Phylogenetic relationships between these 34 isolates and reference strains of the Azorhizobium, Bradyrhizobium, Mesorhizobium, Rhizobium and Ensifer/Sinorhizobium were analysed by using 16S rRNA gene sequences. Twenty-one isolates were related to Mesorhizobium, four isolates to Rhizobium, and nine isolates to Bradyrhizobium. But none of isolates were related to Sinorhizobium/Ensifer and Azorhizobium. Robinia pseudoacacia and Amorpha fruticosa were nodulated by various genotypes of rhizobia out of them, most of the isolates belonged to the genus Mesorhizobium. The isolates from Lespedeza bicolar belonged to diverse genera of Mesorhizobium, Rhizobium, and Bradyrhizobium. The isolates from Maackia amurensis and Lespedeza maximowiezii var. tomentella were phylogenetically related to the genera of Bradyrhizobium. PCR-based RAPD method and phylogenetic analysis of the 16S rRNA results revealed a high phylogenetic diversity of rhizobial strains nodulating leguminous trees in South Korea. Also, the relationships between host and bacterial phylogenies showed that only Robinia pseudoacacia, and Wisteria floribunda have significantly unique branch length than expected by chance based on phylogenetic tree.