L-glutamine supplementation reduced morphological damage in the renal glomerulus of rats with Walker-256 tumor
Acta cir. bras
; Acta cir. bras;38: e383923, 2023. graf, ilus
Article
in En
| LILACS, VETINDEX
| ID: biblio-1513538
Responsible library:
BR68.1
Localization: BR68.1
ABSTRACT
Purpose:
To evaluate the effects of the experimental subcutaneous Walker-256 tumor and L-glutamine supplementation, an antioxidant, on the glomerular morphology of rats.Methods:
Twenty Wistar rats were distributed into four groups (n = 5) control (C); control treated with 2% L-glutamine (CG); rats with Walker-256 tumor (WT); and rats with Walker-256 tumor treated with 2% L-glutamine (WTG). Renal histological samples were submitted to periodic acid-Schiff and Masson's Trichrome staining to analyze glomerular density, morphometry of glomerular components and glomerulosclerosis; and to immunohistochemistry for fibroblast growth factor-2 (FGF-2).Results:
WT showed 50% reduction in body mass gain and cachexia index > 10%, while WTG demonstrated reduction in cachexia (p < 0.05). WT revealed reduction of glomerular density, increase in the glomerular tuft area, mesangial area, matrix in the glomerular tuft, decrease in the urinary space and synechia, and consequently higher glomerulosclerosis (p < 0.05). L-glutamine supplementation in the WTG improved glomerular density, and reduced glomerular tuft area, urinary space, mesangial area, and glomerulosclerosis compared to WT(p < 0.05). WT showed higher collagen area and FGF-2 expression compared to C (p < 0.05). WTG presented lower collagen fibers and FGF-2 expression compared to WT (p < 0.05).Conclusions:
L-glutamine supplementation reduced cachexia and was beneficial for glomerular morphology of the rats, as well as it reduced kidney damage and improved the remaining glomeruli morphology.Key words
Full text:
1
Database:
LILACS
Main subject:
Carcinoma 256, Walker
/
Rats, Wistar
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Glutamine
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Kidney Glomerulus
/
Antioxidants
Language:
En
Journal:
Acta Cir Bras
/
Acta cir. bras
/
Acta cir. bras. (Online)
/
Acta cirúrgica brasileira (Impresso)
Year:
2023
Type:
Article
Affiliation country:
Brazil