Ethyl acetate fraction from Angelica sinensis inhibits IL-1ß-induced rheumatoid synovial fibroblast proliferation and COX-2, PGE2, and MMPs production
Biol. Res
; 47: 1-8, 2014. graf
Article
in En
| LILACS
| ID: biblio-950737
Responsible library:
CL1.1
ABSTRACT
BACKGROUND:
The root of Angelica sinensis (AS), also known as "Dang-gui," was a popular herbal medicine widely used in the treatment of gynecological diseases in China, Korea, and Japan for a long time. This study aimed to determine the effects of ethyl acetate fraction from Angelica sinensis (EAAS) on the interleukin-1ß (IL-1ß)-induced proliferation of rheumatoid arthritis synovial fibroblasts (RASFs), and production of matrix metalloproteinases (MMPs), cyclooxygenase (COX) 2, and prostaglandin E2 (PGE2), involved in articular bone and cartilage destruction, by RASFs.RESULTS:
RASF proliferation was evaluated with cholecystokinin octapeptide (CCK-8) reagent in the presence of IL-1ß with/without EAAS. Expression of MMPs, tissue inhibitor of metalloproteinases-1 (TIMP-1), COXs, PGE2, and intracellular mitogen-activated protein kinase (MAPK) signaling molecules, including p-ERK, p-p38, p-JNK, and NF-κB, were examined using immunoblotting or semi-quantitative reverse transcription-polymerase chain reaction and enzyme-linked immunosorbent assay. EAAS inhibited IL-1ß-induced RASF proliferation; MMP-1, MMP-3, and COX-2 mRNA and protein expressions; and PGE2 production. EAAS also inhibits the phosphorylation of ERK-1/2, p38, and JNK, and activation of NF-κB by IL-1ß.CONCLUSION:
EAAS might be a new therapeutic modality for rheumatoid arthritis management.Key words
Full text:
1
Database:
LILACS
Main subject:
Arthritis, Rheumatoid
/
Bursa, Synovial
/
Inflammation Mediators
/
Angelica sinensis
/
Cell Proliferation
/
Fibroblasts
Language:
En
Journal:
Biol. Res
Year:
2014
Type:
Article
Affiliation country:
South Korea