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Lactose-egg yolk diluent supplemented with N-acetyl-D-glucosamine affect acrosome morphology and motility of frozen-thawed boar sperm.
Yi, Y J; Im, G S; Park, C S.
Affiliation
  • Yi YJ; Division of Animal Science and Resources, Chungnam National University, Daejeon RK 305-764, South Korea.
Anim Reprod Sci ; 74(3-4): 187-94, 2002 Dec 16.
Article in En | MEDLINE | ID: mdl-12417120
ABSTRACT
These experiments were carried out to investigate the effect of N-acetyl-D-glucosamine, and to obtain additional information about the effect of orvus es paste (OEP) and egg yolk concentration in the freezing of boar sperm in the maxi-straw. The highest post-thaw acrosomes of normal apical ridge (NAR) and motility were obtained with 0.025 or 0.05% N-acetyl-D-glucosamine concentration in the first diluent. However, there were no effects of N-acetyl-D-glucosamine among the diluents with or without N-acetyl-D-glucosamine at the second dilution. The N-acetyl-D-glucosamine in the first and second diluents was added at room temperatures (20-23 degrees C) and 5 degrees C, respectively. It is suggested that the temperature of N-acetyl-D-glucosamine addition is important for the effect of boar sperm protection during freezing and thawing. When the 0.05% N-acetyl-D-glucosamine was supplemented in the first diluent, the optimum final OEP content was 0.5%. The optimum content of egg yolk in the diluent with 0.05% N-acetyl-D-glucosamine concentration was 20% and egg yolk was one of the main cryoprotective agents. In conclusion, we found out that the diluent with 0.025 or 0.05% soluble N-acetyl-D-glucosamine in the first diluent, 0.5% final orvus es paste concentration and 20% egg yolk concentration significantly enhanced NAR acrosomes and motility of boar sperm after freezing and thawing.
Subject(s)
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Database: MEDLINE Main subject: Acetylglucosamine / Semen Preservation / Spermatozoa / Swine / Cryopreservation Language: En Journal: Anim Reprod Sci Year: 2002 Type: Article Affiliation country: South Korea
Search on Google
Database: MEDLINE Main subject: Acetylglucosamine / Semen Preservation / Spermatozoa / Swine / Cryopreservation Language: En Journal: Anim Reprod Sci Year: 2002 Type: Article Affiliation country: South Korea