Structural and mutational analysis of Trypanosoma brucei prostaglandin H2 reductase provides insight into the catalytic mechanism of aldo-ketoreductases.

Kilunga, Kubata Bruno; Inoue, Tsuyoshi; Okano, Yousuke; Kabututu, Zakayi; Martin, Samuel K; Lazarus, Michael; Duszenko, Michael; Sumii, Yuichi; Kusakari, Yukiko; Matsumura, Hiroyoshi; Kai, Yasushi; Sugiyama, Shigeru; Inaka, Kouji; Inui, Takashi; Urade, Yoshihiro

Kilunga, Kubata Bruno; Inoue, Tsuyoshi; Okano, Yousuke; Kabututu, Zakayi; Martin, Samuel K; Lazarus, Michael; Duszenko, Michael; Sumii, Yuichi; Kusakari, Yukiko; Matsumura, Hiroyoshi; Kai, Yasushi; Sugiyama, Shigeru; Inaka, Kouji; Inui, Takashi; Urade, Yoshihiro.

Affiliation

Kilunga KB; Department of Molecular Behavioral Biology, Osaka Bioscience Institute, 6-2-4 Furuedai, Suita, Osaka 565-0874, Japan. bkubata@nairobi.mimcom.net

J Biol Chem ; 280(28): 26371-82, 2005 Jul 15.

Article in En | MEDLINE | ID: mdl-15845552

Subject(s)

Hydroxyprostaglandin Dehydrogenases/chemistry; Hydroxyprostaglandin Dehydrogenases/genetics; Oxidoreductases/metabolism; Prostaglandin H2/chemistry; Trypanosoma brucei brucei/metabolism; Amino Acid Sequence; Animals; Catalysis; Catalytic Domain; Circular Dichroism; Citrates/chemistry; Crystallography, X-Ray; DNA Mutational Analysis; DNA, Complementary/metabolism; Evolution, Molecular; Humans; Hydrogen-Ion Concentration; Kinetics; Ligands; Models, Chemical; Models, Molecular; Molecular Sequence Data; Mutagenesis, Site-Directed; Mutation; Protein Binding; Protein Conformation; Protein Structure, Secondary; Protons; Rats; Sequence Homology, Amino Acid; Swine; Tyrosine/chemistry; Ultraviolet Rays

Search on Google

Add to My VHL

XML

PubMed Links

Database: MEDLINE Main subject: Oxidoreductases / Trypanosoma brucei brucei / Hydroxyprostaglandin Dehydrogenases / Prostaglandin H2 Language: En Journal: J Biol Chem Year: 2005 Type: Article Affiliation country: Japan

Search on Google

Add to My VHL

XML

PubMed Links