[Effect of some active Chinese herbal fraction on brain tissue proteomic profile of ischemic mouse].
Zhongguo Zhong Xi Yi Jie He Za Zhi
; 26(6): 526-8, 2006 Jun.
Article
in Zh
| MEDLINE
| ID: mdl-16841670
ABSTRACT
OBJECTIVE:
To study the effect of some active Chinese herbal fraction on protein expression of brain tissue in ischemic mouse with proteomic technique.METHODS:
Ischemia-reperfusion mice were treated with baicalin, geniposide, cholic acid and concha margaritifera respectively for 3 hrs, and then their brain tissue were taken to extract the total protein. Protein expression in ischemic mouse brain was analyzed with surface-enhanced laser desorption/inionation-time of flight-mass spectra (SELDI-TOF-MS) protein-chip.RESULTS:
The four components tested had effect on 3 target proteins at 5373Da, 5707Da and 15103Da, showing the nature of multi-target and with different action on protein expression.CONCLUSION:
Protein-chip is an effective approach for exploring the pharmacological mechanism of Chinese herbal fraction.
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Database:
MEDLINE
Traditional Medicines:
Medicinas_tradicionales_de_asia
/
Medicina_china
Therapeutic Methods and Therapies TCIM:
Terapias_biologicas
Main subject:
Brain
/
Drugs, Chinese Herbal
/
Reperfusion Injury
/
Brain Infarction
/
Phytotherapy
Language:
Zh
Journal:
Zhongguo Zhong Xi Yi Jie He Za Zhi
Year:
2006
Type:
Article