Expression and purification of His-tagged flavonol synthase of Camellia sinensis from Escherichia coli.

Lin, Guang-Zhe; Lian, Yu-Ji; Ryu, Jae-Ha; Sung, Mi-Kyung; Park, Jong-Sug; Park, Hong-Jae; Park, Byung Ki; Shin, Jong-Suh; Lee, Myeong-Sok; Cheon, Choong-Ill

Lin, Guang-Zhe; Lian, Yu-Ji; Ryu, Jae-Ha; Sung, Mi-Kyung; Park, Jong-Sug; Park, Hong-Jae; Park, Byung Ki; Shin, Jong-Suh; Lee, Myeong-Sok; Cheon, Choong-Ill.

Affiliation

Lin GZ; Department of Biological Science, Sookmyung Women's University, Hyochangwon-gil 52, Yongsan-gu, Seoul 140-742, Republic of Korea.

Protein Expr Purif ; 55(2): 287-92, 2007 Oct.

Article in En | MEDLINE | ID: mdl-17629496

Subject(s)

Camellia sinensis/enzymology; Escherichia coli/genetics; Oxidoreductases/genetics; Plant Proteins/genetics; Amino Acid Sequence; Base Sequence; Chromatography, High Pressure Liquid; DNA Primers; DNA, Complementary; Electrophoresis, Polyacrylamide Gel; Molecular Sequence Data; Oxidoreductases/chemistry; Oxidoreductases/isolation & purification; Plant Proteins/chemistry; Plant Proteins/isolation & purification; Sequence Homology, Amino Acid

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Database: MEDLINE Main subject: Oxidoreductases / Plant Proteins / Camellia sinensis / Escherichia coli Language: En Journal: Protein Expr Purif Year: 2007 Type: Article

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Database: MEDLINE Main subject: Oxidoreductases / Plant Proteins / Camellia sinensis / Escherichia coli Language: En Journal: Protein Expr Purif Year: 2007 Type: Article