Characterization of the trace-level impurities in the bulk drug citalopram by high-performance liquid chromatography/tandem multistage mass spectrometry.
Rapid Commun Mass Spectrom
; 21(17): 2889-94, 2007.
Article
in En
| MEDLINE
| ID: mdl-17663502
ABSTRACT
Five trace impurities were detected in the bulk drug citalopram using high-performance liquid chromatography with UV detection. A simple and sensitive method suitable for liquid chromatography tandem multistage mass spectrometry (HPLC/MS(n)) analysis was developed. Using this method, the fragmentation behavior of citalopram and the impurities was investigated. Four impurities were rapidly characterized, and an unknown impurity was elucidated as 3-(3-dimethylaminopropyl)-N-(1-(3-dimethylaminopropyl)-1-(4-fluorophenyl)-1,3-dihydroisobenzofuran-5-yl)-3-(4-fluorophenyl)-1,3-dihydroisobenzofuran-5-carboxamide on the basis of the MS(n) and exact mass evidence, and the proposed structure was further confirmed by nuclear magnetic resonance (NMR) experiments after preparative isolation.
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Database:
MEDLINE
Main subject:
Citalopram
/
Drug Contamination
/
Chromatography, High Pressure Liquid
/
Spectroscopy, Fourier Transform Infrared
/
Spectrometry, Mass, Electrospray Ionization
/
Drug Evaluation, Preclinical
Type of study:
Diagnostic_studies
Language:
En
Journal:
Rapid Commun Mass Spectrom
Year:
2007
Type:
Article
Affiliation country:
China