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The expression of classical swine fever virus structural protein E2 gene in tobacco chloroplasts for applying chloroplasts as bioreactors.
Shao, Hong-Bo; He, Dong-Mei; Qian, Kai-Xian; Shen, Gui-Fang; Su, Zhong-Liang.
Affiliation
  • Shao HB; Binzhou University, Binzhou 256603, China; Institute of Soil and Water Conservation, Chinese Academy of Sciences & Water Resources Ministry (CAS&WRM), Yangling 712100, China. shaohongbochu@126.com
C R Biol ; 331(3): 179-84, 2008 Mar.
Article in En | MEDLINE | ID: mdl-18280983
ABSTRACT
It has been reported that genes encoding antigens of bacterial and viral pathogens can be expressed in plants and are shown to induce protection antibodies. The structural protein E2 of classical swine fever virus (CSFV), which has been shown to carry critical epitopes, has been expressed in different systems. Here, we report the expression of CFSV E2 gene in tobacco chloroplasts. Mice immunized with leaf extracts elicited specific antibodies. This indicated that the expressed E2 proteins had a certain degree of immunogenicity. To our knowledge, this is the first report showing induction of protective antibody in response to classical swine fever virus (CSFV) by immunization with antigen protein E2 expressed in tobacco chloroplasts, which will open a new way to protection from CSFV by plant chloroplasts as bioreactors.
Subject(s)

Full text: 1 Database: MEDLINE Main subject: Nicotiana / Gene Expression / Chloroplasts / Viral Envelope Proteins / Bioreactors / Classical Swine Fever Virus Language: En Journal: C R Biol Year: 2008 Type: Article Affiliation country: China

Full text: 1 Database: MEDLINE Main subject: Nicotiana / Gene Expression / Chloroplasts / Viral Envelope Proteins / Bioreactors / Classical Swine Fever Virus Language: En Journal: C R Biol Year: 2008 Type: Article Affiliation country: China