Cloning and characterization of a new cold-active lipase from a deep-sea sediment metagenome.

Jeon, Jeong Ho; Kim, Jun-Tae; Kim, Yun Jae; Kim, Hyung-Kwoun; Lee, Hyun Sook; Kang, Sung Gyun; Kim, Sang-Jin; Lee, Jung-Hyun

Jeon, Jeong Ho; Kim, Jun-Tae; Kim, Yun Jae; Kim, Hyung-Kwoun; Lee, Hyun Sook; Kang, Sung Gyun; Kim, Sang-Jin; Lee, Jung-Hyun.

Affiliation

Jeon JH; Marine Biotechnology Center, Korea Ocean Research and Development Institute, Ansan P.O. Box 29, Seoul 425-600, South Korea.

Appl Microbiol Biotechnol ; 81(5): 865-74, 2009 Jan.

Article in En | MEDLINE | ID: mdl-18773201

Subject(s)

Geologic Sediments/microbiology; Lipase/genetics; Lipase/metabolism; Caprylates/metabolism; Chromatography, Affinity; Cloning, Molecular; Enzyme Inhibitors/pharmacology; Enzyme Stability; Escherichia coli; Gene Expression; Hydrogen-Ion Concentration; Lipase/chemistry; Molecular Sequence Data; Octoxynol/pharmacology; Olive Oil; Phylogeny; Plant Oils/metabolism; Polysorbates/pharmacology; Recombinant Fusion Proteins/biosynthesis; Recombinant Fusion Proteins/genetics; Recombinant Fusion Proteins/isolation & purification; Sequence Analysis, DNA; Sequence Homology, Amino Acid; Substrate Specificity; Temperature; Triglycerides/metabolism

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Full text: 1 Database: MEDLINE Main subject: Geologic Sediments / Lipase Language: En Journal: Appl Microbiol Biotechnol Year: 2009 Type: Article Affiliation country: South Korea

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