Puerarin protects against high glucose-induced acute vascular dysfunction: role of heme oxygenase-1 in rat thoracic aorta.
Vascul Pharmacol
; 50(3-4): 110-5, 2009.
Article
in En
| MEDLINE
| ID: mdl-19073281
ABSTRACT
OBJECTIVE:
To investigate the antioxidant and vascular protective effect of puerarin, an isoflavone glycoside known in traditional Chinese medicine on vascular reactivity subsequent to high glucose stress.METHODS:
The thoracic aortic rings with or without endothelium from male SD rats were mounted in an organ bath. Isometric contraction of aortic rings was measured. HO-1 protein expression and HO activity were also evaluated.RESULTS:
(1) After incubation with 44 mmol/L of high glucose for 2 or 4 h, the vascular contraction responses to phenylephrine (PE) and relaxation response to acetylcholine (Ach) decreased in an endothelium-dependent manner; (2) Coincubation with puerarin (10(-10)-10(-8) mol/L) and high glucose, the high glucose-induced vasoconstriction and vasodilation dysfunction was partly inhibited in a dose-dependent manner; (3) Puerarin increased the HO-1 protein expression and HO activity of thoracic aorta. ZnPP (an inhibitor of heme oxygenase-1) offset the protective effect of puerarin.CONCLUSION:
Puerarin could alleviate the high glucose-induced acute endothelium-dependent vascular dysfunction in rat aortic rings. HO-1 activity was proposed as a mechanism to account for the protection of vascular responses by puerarin.
Full text:
1
Database:
MEDLINE
Traditional Medicines:
Medicinas_tradicionales_de_asia
/
Medicina_china
Main subject:
Aorta, Thoracic
/
Vasoconstriction
/
Vasodilation
/
Vasodilator Agents
/
Heme Oxygenase-1
/
Glucose
/
Isoflavones
Language:
En
Journal:
Vascul Pharmacol
Year:
2009
Type:
Article
Affiliation country:
China