Identification of inhibitors of vacuolar proton-translocating ATPase pumps in yeast by high-throughput screening flow cytometry.

Johnson, Rebecca M; Allen, Chris; Melman, Sandra D; Waller, Anna; Young, Susan M; Sklar, Larry A; Parra, Karlett J

Johnson, Rebecca M; Allen, Chris; Melman, Sandra D; Waller, Anna; Young, Susan M; Sklar, Larry A; Parra, Karlett J.

Affiliation

Johnson RM; Department of Biochemistry and Molecular Biology, University of New Mexico School of Medicine, Albuquerque, NM, USA.

Anal Biochem ; 398(2): 203-11, 2010 Mar 15.

Article in En | MEDLINE | ID: mdl-20018164

Subject(s)

Drug Evaluation, Preclinical/methods; Enzyme Inhibitors/pharmacology; Flow Cytometry; High-Throughput Screening Assays; Saccharomyces cerevisiae/enzymology; Vacuolar Proton-Translocating ATPases/antagonists & inhibitors; Yeasts/enzymology; Fluoresceins/chemistry; Hydrogen-Ion Concentration; Macrolides/pharmacology; Spectrometry, Fluorescence; Vacuoles; Yeasts/cytology; Yeasts/drug effects

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Full text: 1 Database: MEDLINE Main subject: Saccharomyces cerevisiae / Yeasts / Vacuolar Proton-Translocating ATPases / Drug Evaluation, Preclinical / Enzyme Inhibitors / High-Throughput Screening Assays / Flow Cytometry Type of study: Diagnostic_studies / Screening_studies Language: En Journal: Anal Biochem Year: 2010 Type: Article Affiliation country: United States

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