Efficient production and purification of functional bacteriorhodopsin with a wheat-germ cell-free system and a combination of Fos-choline and CHAPS detergents.

Genji, Takahisa; Nozawa, Akira; Tozawa, Yuzuru

Genji, Takahisa; Nozawa, Akira; Tozawa, Yuzuru.

Affiliation

Genji T; Cell-Free Science and Technology Research Center and Venture Business Laboratory, Ehime University, 3 Bunkyo-cho, Matsuyama, Ehime 790-8577, Japan.

Biochem Biophys Res Commun ; 400(4): 638-42, 2010 Oct 01.

Article in En | MEDLINE | ID: mdl-20807510

Subject(s)

Bacteriorhodopsins/biosynthesis; Bacteriorhodopsins/isolation & purification; Cholic Acids/chemistry; Detergents/chemistry; Phosphorylcholine/analogs & derivatives; Protein Biosynthesis; Bacteriorhodopsins/antagonists & inhibitors; Cell-Free System; Cholic Acids/pharmacology; Detergents/pharmacology; Germ Cells; Micelles; Phosphorylcholine/chemistry; Phosphorylcholine/pharmacology; Triticum

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Full text: 1 Database: MEDLINE Main subject: Phosphorylcholine / Protein Biosynthesis / Bacteriorhodopsins / Cholic Acids / Detergents Language: En Journal: Biochem Biophys Res Commun Year: 2010 Type: Article Affiliation country: Japan

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