Simultaneous determination of nucleosides and their bases in Cordyceps sinensis and its substitutes by matrix solid-phase dispersion extraction and HPLC.
J Sep Sci
; 36(14): 2348-57, 2013 Jul.
Article
in En
| MEDLINE
| ID: mdl-23677705
ABSTRACT
Nine nucleosides and nucleobases, including uracil, adenine, thymine, uridine, adenosine, thymidine, cytidine, guanosine, and cordycepin in natural Cordyceps sinensis, cultured Cordyceps mycelia, and Cordyceps fruiting bodies were extracted by matrix solid-phase dispersion (MSPD) and determined by HPLC. The experimental conditions for the MSPD extraction were optimized. Florisil was used as dispersant, petroleum ether as washing solvent, and methanol as elution solvent. The Florisil-to-sample ratio was selected to be 41 and no additional clean-up sorbent was needed. The calibration curves had good linear relationships (r > 0.9997). The LOD and LOQ were in the range of 12ï½79 and 41ï½265 ng/mL, respectively. The intra- and interday precision were lower than 8.3%. The recoveries were between 61.5 and 93.2%. The present method consumed less sample compared with ultrasonic extraction and heating reflux extraction (HRE). The extraction yields obtained by using the present method are much higher than those obtained by UE and comparable to those obtained by HRE.
Key words
Full text:
1
Database:
MEDLINE
Main subject:
Plant Extracts
/
Chromatography, High Pressure Liquid
/
Cordyceps
/
Solid Phase Extraction
/
Nucleosides
/
Nucleotides
Type of study:
Evaluation_studies
Language:
En
Journal:
J Sep Sci
Year:
2013
Type:
Article