Qualitative and quantitative analysis of seven oligosaccharides in Morinda officinalis using double-development HPTLC and scanning densitometry.
Biomed Mater Eng
; 24(1): 953-60, 2014.
Article
in En
| MEDLINE
| ID: mdl-24211984
ABSTRACT
The quality of Morindaofficinalis, which has been used as a Yang-tonic agent for a long time in China, can be evaluated. A double-development high performance thin layer chromatography (HPTLC) method has been established to simultaneously analyze quality and quantity of seven inulin-type oligosaccharides (DP=3-9) in Morindaofficinalis. The chromatography was performed on a silica gel 60 plate with the 7521 proportion (v/v) of n-butanol-isopropanol-water-acetic acid for the first and second developments, respectively. The bands were visualized by the reaction with aniline-diphenylamine-phosphoric acid solution and analyzed by densitometric TLC at 540 nm. Quantification of seven oligosaccharides was achieved by densitometry at 540 nm. The investigated standard sugar had good linearity (R2>0.99) within test ranges. The amounts of seven oligosaccharides were calculated by the relative correction factor (RCF). Therefore, the developed TLC method could be used for quality control of Morindaofficinalis.
Key words
Full text:
1
Database:
MEDLINE
Main subject:
Oligosaccharides
/
Chromatography, High Pressure Liquid
/
Morinda
/
Densitometry
Type of study:
Diagnostic_studies
/
Qualitative_research
Country/Region as subject:
Asia
Language:
En
Journal:
Biomed Mater Eng
Year:
2014
Type:
Article
Affiliation country:
China