Detection of glycoalkaloids using disposable biosensors based on genetically modified enzymes.
Anal Biochem
; 457: 85-90, 2014 Jul 15.
Article
in En
| MEDLINE
| ID: mdl-24747413
ABSTRACT
In this work we present a rapid, selective, and highly sensitive detection of α-solanine and α-chaconine using cholinesterase-based sensors. The high sensitivity of the devices is brought by the use of a genetically modified acetylcholinesterase (AChE), combined with a one-step detection method based on the measurement of inhibition slope. The selectivity was obtained by using butyrylcholinesterase (BChE), an enzyme able to detect these two toxins with differential inhibition kinetics. The enzymes were immobilized via entrapment in PVA-AWP polymer directly on the working electrode surface. The analysis of the resulting inhibition slope was performed employing linear regression function included in Matlab. The high toxicity of α-chaconine compared to α-solanine due to a better affinity to the active site was proved. The inhibition of glycoalkaloids (GAs) mixture was performed over AChE enzyme wild-type AChE and BChE biosensors resulting in the detection of synergism effect. The developed method allows the detection of (GAs) at 50 ppb in potato matrix.
Key words
Full text:
1
Database:
MEDLINE
Main subject:
Acetylcholinesterase
/
Solanine
/
Butyrylcholinesterase
/
Biosensing Techniques
/
Disposable Equipment
Type of study:
Diagnostic_studies
Language:
En
Journal:
Anal Biochem
Year:
2014
Type:
Article