[Establishment of transformation system of Lonicera macranthoides mediated by Agrobacterium tumefaciens].

ABSTRACT

OBJECTIVE: Based on the system of regeneration,the genetic transformation system of Lonicera macranthoides was established. METHODS: Tissue culture method of seedlings, Agrobacterium tumefaciens mediated transformation method of explants, report gene was detected by gus staining and PCR. RESULTS: The efficient transformation time was 8 minutes of infection. The good transformation rate was gained with the kanamycin 35 mg/L and cefotaxime 600 mg/L. The concentration of kanamycin had a leading effect on bud differentiation between two antibiotics, and bud induction rate reached extremely significant difference. Results of gus staining and PCR proved that the gus gene was integrated into Lonicera macranthoides genome. CONCLUSION: The genetic transformation system of Lonicera macranthoides leaves mediated by Agrobacterium tumefaciens EHAlO5 was established for the first time.