Preparation of lysine-coated magnetic Fe2O3 nanoparticles and influence on viability of A549 lung cancer cells.
Asian Pac J Cancer Prev
; 15(20): 8981-5, 2014.
Article
in En
| MEDLINE
| ID: mdl-25374240
ABSTRACT
OBJECTIVE:
To explore the effect of lysine-coated oxide magnetic nanoparticles (Lys@MNPs) on viability and apoptosis of A549 lung cancer cells.METHODS:
Transmission electron microscopy (TEM), vibrating sample magnetometer (VSM) and Zeta potentiometric analyzer were employed to characterize Lys@MNPs. Then Lys@MNPs and lung cancer A549 cells were co-cultured to study the effect of Lys@MNPs on cell viability and apoptosis. The pathway of Lys@MNPs entering A549 cells was detected by TEM and cell imaging by 1.5 T MRI.RESULTS:
Lys@MNPs were 10.2 nm in grain diameter, characterized by small size, positive charge, and superparamagnetism. Under low-dose concentration of Lys@MNPs (<40 µg/mL), the survival rate of A549 cells was decreased but remained higher than 95% while under high-dose concentration (100 µg/mL), the survival ratewas still higher than 80%, which suggested Lys@MNPs had limited influence on the viability of A549 cells, with good biocompatibility and and no induction of apoptosis. Moreover, high affinity for cytomembranes, was demonstrated presenting good imaging effects.CONCLUSION:
Lys@MNPs can be regarded as a good MRI negative contrast agents, with promising prospects in biomedicine.
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Database:
MEDLINE
Main subject:
Ferric Compounds
/
Apoptosis
/
Cell Proliferation
/
Magnetite Nanoparticles
/
Lung Neoplasms
/
Lysine
Language:
En
Journal:
Asian Pac J Cancer Prev
Year:
2014
Type:
Article