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MiR-219 Protects Against Seizure in the Kainic Acid Model of Epilepsy.
Zheng, Honghua; Tang, Rong; Yao, Yi; Ji, Zhilin; Cao, Yuanyuan; Liu, Zhaoji; Peng, Feng; Wang, Wenjie; Can, Dan; Xing, Huiqin; Bu, Guojun; Xu, Huaxi; Zhang, Yun-Wu; Zheng, Weihong.
Affiliation
  • Zheng H; Fujian Provincial Key Laboratory of Neurodegenerative Disease and Aging Research, Institute of Neuroscience, Medical College, Xiamen University, Xiamen, 361005, Fujian Province, China.
  • Tang R; Department of Basic Medicine, Medical College, Xiamen University, Xiamen, 361005, Fujian Province, China.
  • Yao Y; Department of Neurology, Affiliated Zhongshan Hospital, Xiamen University, Xiamen, 361004, Fujian Province, China.
  • Ji Z; Epilepsy Research Center, Department of Neurosurgery, Affiliated Chenggong Hospital, Xiamen University, Xiamen, 361004, Fujian Province, China.
  • Cao Y; Fujian Provincial Key Laboratory of Neurodegenerative Disease and Aging Research, Institute of Neuroscience, Medical College, Xiamen University, Xiamen, 361005, Fujian Province, China.
  • Liu Z; Department of Basic Medicine, Medical College, Xiamen University, Xiamen, 361005, Fujian Province, China.
  • Peng F; Department of Neurology, Affiliated Zhongshan Hospital, Xiamen University, Xiamen, 361004, Fujian Province, China.
  • Wang W; Department of Neurology, Affiliated Zhongshan Hospital, Xiamen University, Xiamen, 361004, Fujian Province, China.
  • Can D; Department of Neurology, Affiliated Zhongshan Hospital, Xiamen University, Xiamen, 361004, Fujian Province, China.
  • Xing H; Department of Neurology, Affiliated Zhongshan Hospital, Xiamen University, Xiamen, 361004, Fujian Province, China.
  • Bu G; Fujian Provincial Key Laboratory of Neurodegenerative Disease and Aging Research, Institute of Neuroscience, Medical College, Xiamen University, Xiamen, 361005, Fujian Province, China.
  • Xu H; Fujian Provincial Key Laboratory of Neurodegenerative Disease and Aging Research, Institute of Neuroscience, Medical College, Xiamen University, Xiamen, 361005, Fujian Province, China.
  • Zhang YW; Fujian Provincial Key Laboratory of Neurodegenerative Disease and Aging Research, Institute of Neuroscience, Medical College, Xiamen University, Xiamen, 361005, Fujian Province, China.
  • Zheng W; Fujian Provincial Key Laboratory of Neurodegenerative Disease and Aging Research, Institute of Neuroscience, Medical College, Xiamen University, Xiamen, 361005, Fujian Province, China.
Mol Neurobiol ; 53(1): 1-7, 2016 Jan.
Article in En | MEDLINE | ID: mdl-25394384
Emerging evidence indicates that certain microRNAs (miRNAs) play important roles in epileptogenesis. MiR-219 is a brain-specific miRNA and has been shown to negatively regulate the function of N-methyl-D-aspartate (NMDA) receptors by targeting Ca(2+)/calmodulin-dependent protein kinase II (CaMKII)γ. Herein, we found that the level of miR-219 was decreased in both the kainic acid (KA)-induced epilepsy model and in cerebrospinal fluid specimens of epilepsy patients. Importantly, silencing of miR-219 by its antagomir in vivo resulted in seizure behaviors, abnormal cortical electroencephalogram (EEG) recordings in the form of high-amplitude and high-frequency discharges, and increased levels of CaMKIIγ and an NMDA receptor component, NR1, in a pattern similar to that found in KA-treated mice. Moreover, treatments with the miR-219 agomir in vivo alleviated seizures, abnormal EEG recordings, and decreased levels of CaMKIIγ and NR1 in KA-treated mice. Furthermore, treatment with MK-801, an antagonist of NMDA receptors, significantly alleviated abnormal EEG recordings induced by miR-219 antagomir. Together, these results demonstrate that miR-219 plays a crucial role in suppressing seizure formation in experimental models of epilepsy through modulating the CaMKII/NMDA receptor pathway and that miR-219 supplement may be a potential anabolic strategy for ameliorating epilepsy.
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Full text: 1 Database: MEDLINE Main subject: Seizures / Brain / Receptors, N-Methyl-D-Aspartate / MicroRNAs / Neurons Type of study: Diagnostic_studies / Prognostic_studies Language: En Journal: Mol Neurobiol Year: 2016 Type: Article Affiliation country: China

Full text: 1 Database: MEDLINE Main subject: Seizures / Brain / Receptors, N-Methyl-D-Aspartate / MicroRNAs / Neurons Type of study: Diagnostic_studies / Prognostic_studies Language: En Journal: Mol Neurobiol Year: 2016 Type: Article Affiliation country: China