A Sensitive and Specific Indirect Competitive Enzyme-Linked Immunosorbent Assay for Detection of Paeoniflorin and Its Application in Pharmacokinetic Interactions between Paeoniflorin and Glycyrrhizinic Acid.
Planta Med
; 81(9): 765-70, 2015 Jun.
Article
in En
| MEDLINE
| ID: mdl-25856441
ABSTRACT
This study aimed to develop an indirect competitive enzyme-linked immunosorbent assay based on monoclonal antibodies against paeoniflorin to study the effects of different doses of glycyrrhizinic acid on the pharmacokinetics of paeoniflorin in mice. An anti-paeoniflorin monoclonal antibody was produced from a hybridoma created through a fusion of splenocytes immunized with paeoniflorin-bovine serum albumin and conjugated with the hypoxanthine-aminopterin-thymidine-sensitive mouse myeloma cell line SP2/0. The resultant antibody was used to develop and validate a rapid, specific and sensitive, indirect competitive enzyme-linked immunosorbent assay for the measurement of paeoniflorin (linear range 4.8-312.5 ngâ·âmL(-1)). The intraday and interday precision values of the indirect competitive ELISA method were well within the recommended range (≤ 10â%), and the recovery rate was, on average, 101.13â%. Pharmacokinetic parameters obtained from mouse blood samples at various intervals following the oral administration of paeoniflorin and glycyrrhizic acid at three doses (1ââ0.3, 1ââ1, 1ââ3, respectively) demonstrated that the highest dose of glycyrrhizic acid inhibits the absorption of paeoniflorin.
Full text:
1
Database:
MEDLINE
Main subject:
Enzyme-Linked Immunosorbent Assay
/
Glycyrrhizic Acid
/
Monoterpenes
/
Glucosides
Type of study:
Diagnostic_studies
Language:
En
Journal:
Planta Med
Year:
2015
Type:
Article
Affiliation country:
China