Kinetic characterization of oxyresveratrol as a tyrosinase substrate.
IUBMB Life
; 67(11): 828-36, 2015 Nov.
Article
in En
| MEDLINE
| ID: mdl-26450473
Oxyresveratrol is a stilbenoid described as a powerful inhibitor of tyrosinase and proposed as skin-whitening and anti-browning agent. However, the enzyme is capable of acting on it, considering it as a substrate, as it has been proved in the case of its analogous resveratrol. Tyrosinase hydroxylates the oxyresveratrol to an o-diphenol and oxidizes the latter to an o-quinone, which finally isomerizes to p-quinone. For these reactions to take place the presence of the Eox (oxy-tyrosinase) form is necessary. The kinetic analysis of the proposed mechanism has allowed the kinetic characterization of this molecule as a substrate of tyrosinase, affording a catalytic constant of 5.39 ± 0.21 sec(-1) and a Michaelis constant of 8.65 ± 0.73 µM.
Key words
Full text:
1
Database:
MEDLINE
Main subject:
Stilbenes
/
Fungal Proteins
/
Plant Extracts
/
Monophenol Monooxygenase
Language:
En
Journal:
IUBMB Life
Year:
2015
Type:
Article
Affiliation country:
Spain