A Liquid Chromatography-Tandem Mass Spectrometry Method for Simultaneous Quantitation of 10 Bioactive Components in Rhus verniciflua Extracts.
J Chromatogr Sci
; 54(3): 390-6, 2016 Mar.
Article
in En
| MEDLINE
| ID: mdl-26499118
ABSTRACT
The purpose of this study was to develop a simultaneous method to quantify 10 bioactive compounds in Rhus verniciflua extracts using high-performance liquid chromatography-tandem mass spectrometry. The chromatographic separation was performed using a C18 column under gradient elution with 0.1% formic acid and acetonitrile as the mobile phase solvents. The analytes were detected in the negative-ion mode using multiple-reaction monitoring detection with an electrospray ionization interface. The calibration curves for all the analytes showed good linearity (r(2) >0.997) over the concentration range of 1-1,000 ng/mL. The recovery values were within 89.53-110.14%, and the intra- and interday coefficients of variation were <4.86% for all the tested compounds. The developed method was successfully applied to a quality assessment of the R. verniciflua extract samples.
Full text:
1
Database:
MEDLINE
Main subject:
Flavonoids
/
Plant Extracts
/
Chromatography, Liquid
/
Rhus
/
Tandem Mass Spectrometry
/
Gallic Acid
Type of study:
Diagnostic_studies
Language:
En
Journal:
J Chromatogr Sci
Year:
2016
Type:
Article