Comparison between the additive effects of diluted (rFSH) and diluted/dynamized (FSH 6 cH) recombinant follicle-stimulating hormone on the in vitro culture of ovine preantral follicles enclosed in ovarian tissue.
Complement Ther Med
; 25: 39-44, 2016 Apr.
Article
in En
| MEDLINE
| ID: mdl-27062946
ABSTRACT
OBJECTIVE:
This study compared 2 types of recombinant follicle stimulating hormone (rFSH) diluted and diluted/dynamized, on in vitro development of ovine follicles.METHODS:
In experiment 1, ovarian fragments were cultured for 1 or 7 days in α-MEM(+) in the absence or presence of different concentrations of diluted rFSH to determine the best concentration. In experiment 2, the effect of diluted and diluted/dynamized rFSH (rFSH 6 cH--ultradiluted and succussioned), alone or in combination, was studied.RESULTS:
In experiment 1, compared to control, 50ng/mL of diluted rFSH induced higher rates of follicular survival after 7 days of culture and higher percentages of growing follicles at day 1 of culture (P<0.05). In experiment 2, compared to control, diluted/dynamized rFSH induced higher follicular diameter and survival rate after 7 days and early follicle activation at day 1 of culture (P<0.05). Compared to diluted rFSH, diluted/dynamized rFSH induced higher rates of follicle activation at day 1 of culture (P<0.05).CONCLUSION:
In conclusion, compared to the control medium, diluted/dynamized rFSH promoted survival and early activation of follicles, while diluted rFSH promoted higher activation later in the culture. Thus, diluted/dynamized rFSH may be used as an alternative to diluted rFSH for the in vitro culture of ovine preantral follicles.Key words
Full text:
1
Database:
MEDLINE
Main subject:
Recombinant Proteins
/
Follicle Stimulating Hormone
/
Ovarian Follicle
Language:
En
Journal:
Complement Ther Med
Year:
2016
Type:
Article