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Standards for Cell Line Authentication and Beyond.
Almeida, Jamie L; Cole, Kenneth D; Plant, Anne L.
Affiliation
  • Almeida JL; Biosystems and Biomaterials Division, The National Institute of Standards and Technology, Gaithersburg, Maryland, United States of America.
  • Cole KD; Biosystems and Biomaterials Division, The National Institute of Standards and Technology, Gaithersburg, Maryland, United States of America.
  • Plant AL; Biosystems and Biomaterials Division, The National Institute of Standards and Technology, Gaithersburg, Maryland, United States of America.
PLoS Biol ; 14(6): e1002476, 2016 06.
Article in En | MEDLINE | ID: mdl-27300367
ABSTRACT
Different genomic technologies have been applied to cell line authentication, but only one method (short tandem repeat [STR] profiling) has been the subject of a comprehensive and definitive standard (ASN-0002). Here we discuss the power of this document and why standards such as this are so critical for establishing the consensus technical criteria and practices that can enable progress in the fields of research that use cell lines. We also examine other methods that could be used for authentication and discuss how a combination of methods could be used in a holistic fashion to assess various critical aspects of the quality of cell lines.
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Full text: 1 Database: MEDLINE Main subject: Microsatellite Repeats / Gene Expression Profiling / Polymorphism, Single Nucleotide / Genotyping Techniques Type of study: Guideline Language: En Journal: PLoS Biol Year: 2016 Type: Article Affiliation country: United States
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Add to My VHL
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Full text: 1 Database: MEDLINE Main subject: Microsatellite Repeats / Gene Expression Profiling / Polymorphism, Single Nucleotide / Genotyping Techniques Type of study: Guideline Language: En Journal: PLoS Biol Year: 2016 Type: Article Affiliation country: United States