Qualitative and quantitative analyses of Compound Danshen extract based on 1H NMR method and its application for quality control.
J Pharm Biomed Anal
; 131: 183-187, 2016 Nov 30.
Article
in En
| MEDLINE
| ID: mdl-27596830
ABSTRACT
In this study, a new approach using 1H NMR spectroscopy combined with chemometrics method was developed for qualitative and quantitative analyses of extracts of Compound Danshen Dripping Pills (CDDP). For the qualitative analysis, some metabolites presented in Compound Danshen extract (CDE, extraction intermediate of CDDP) were detected, including phenolic acids, saponins, saccharides, organic acids and amino acids, by the proposed 1H NMR method, and metabolites profiles were further analyzed by selected chemometrics algorithms to define the threshold values for product quality evaluation. Moreover, three main phenolic acids (danshensu, salvianolic acid B, and procatechuic aldehyde) in CDE were determined simultaneously, and method validation in terms of linearity, precision, repeatability, accuracy, and stability of the dissolved target compounds in solution was performed. The average recoveries varied between 84.20% and 110.75% while the RSDs were below 6.34% for the three phenolic acids. This 1H NMR method offers an integral view of the extract composition, allows the qualitative and quantitative analysis of CDDP, and has the potential to be a supplementary tool to UPLC/HPLC for quality assessment of Chinese herbal medicines.
Key words
Full text:
1
Database:
MEDLINE
Traditional Medicines:
Medicinas_tradicionales_de_asia
/
Medicina_china
Main subject:
Quality Control
/
Drugs, Chinese Herbal
/
Proton Magnetic Resonance Spectroscopy
Type of study:
Qualitative_research
Language:
En
Journal:
J Pharm Biomed Anal
Year:
2016
Type:
Article
Affiliation country:
China