Biochemical characterization, anti-inflammatory properties and ulcerogenic traits of some cold-pressed oils in experimental animals.
Pharm Biol
; 55(1): 740-748, 2017 Dec.
Article
in En
| MEDLINE
| ID: mdl-28056572
ABSTRACT
CONTEXT Cold-pressed oils (CPO) are commercially available in the market and characterized by their health-promoting properties. OBJECTIVE:
Clove oil (CLO), coriander seed oil (COO) and black cumin oil (BCO) were evaluated for their bioactive lipids. Pharmacological screening was performed to evaluate acute toxicity, anti-inflammatory and ulcerogenic effects as well as histopathological changes in tissues of albino rats fed with CPO. MATERIALS ANDMETHODS:
Fatty acids, tocols and total phenolics were analyzed. The acute toxicity test for each CPO was estimated during 14 d. Carrageenan-induced rat paw oedema was used for assessment of anti-inflammatory activity of CPO. Animals were fasted overnight, and via oral gavage given indomethacin (10 mg/kg) or CPO (400 mg/kg) to investigate ulcerogenecity. Histopathological changes in liver, kidney, heart, spleen and stomach were screened.RESULTS:
Amounts of α-, ß-, γ- and δ-tocopherols in CLO were 1495, 58, 4177 and 177 mg/kg oil, respectively. In COO, α, ß, γ and δ-tocopherols were 10.0, 18.2, 5.1 and 34.8%, respectively. In BCO, ß-tocotrienol was the main constituent. CLO, COO and BCO contained 4.6, 4.2 and 3.6 mg GAE/g, respectively. Acute toxicity test determined that 400 mg/kg of CPO to be used. In the carrageenan model of inflammation, pretreatment of rats with indomethacin (10 mg/kg) or CLO (400 mg/kg) induced a significant (p < 0.05) reduction by 31.3 and 27.4%, respectively, in rat paw oedema as compared with the carrageenan-treated group. Indomethacin induced a significant ulcerogenic effect with an ulcer index of 19. Oral treatment of CPO showed no ulcerogenic effect, wherein no histopathological changes were observed.CONCLUSIONS:
CPO, particularly CLO, could minimize acute inflammation.Key words
Full text:
1
Database:
MEDLINE
Main subject:
Stomach Ulcer
/
Plant Oils
/
Anti-Inflammatory Agents
Language:
En
Journal:
Pharm Biol
Year:
2017
Type:
Article
Affiliation country:
Egypt