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The effect of combined photobiomodulation and metformin on open skin wound healing in a non-genetic model of type II diabetes.
Asghari, Mohammadali; Kanonisabet, Ali; Safakhah, Mandanad; Azimzadeh, Zahra; Mostafavinia, Ataroalsadat; Taheri, Soudabeh; Amini, Abdollah; Ghorishi, Seyed Kamran; JalaliFiroozkohi, Reza; Bayat, Sahar; Bayat, Mohammad.
Affiliation
  • Asghari M; School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran. Electronic address: mohammad.a.asghari@gmail.com.
  • Kanonisabet A; School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran. Electronic address: alikanoonisabet@yahoo.com.
  • Safakhah M; School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran. Electronic address: mandanasafakhahmd@gmail.com.
  • Azimzadeh Z; School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran. Electronic address: zahahraazimzadehmd@gmail.com.
  • Mostafavinia A; Department of Biology and Anatomical Sciences, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran. Electronic address: a.mostafavinia@gmail.com.
  • Taheri S; Department of Microbiology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran. Electronic address: so_taheri@yahoo.co.uk.
  • Amini A; Department of Biology and Anatomical Sciences, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran. Electronic address: d.amini2008@yahoo.com.
  • Ghorishi SK; Department of Statistics, Qom University, Qom, Iran. Electronic address: atty_ghoreishi@yahoo.com.
  • JalaliFiroozkohi R; School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran. Electronic address: reza.jalali.7082@gmail.com.
  • Bayat S; Nicholas Senn High School, Chicago IL, USA. Electronic address: sbayat1@cps.edu.
  • Bayat M; Cellular and Molecular Biology Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran. Electronic address: mohbayat@sbmu.ac.ir.
J Photochem Photobiol B ; 169: 63-69, 2017 Apr.
Article in En | MEDLINE | ID: mdl-28282557
This study intended to examine the combined influences of photobiomodulation (PBM) and metformin on the microbial flora and biomechanical parameters of wounds in a non-genetic model of type II diabetes mellitus (TII DM). We induced a non-genetic model of TII DM in 20 rats by feeding them a 10% fructose solution for 2weeks followed by an injection of streptozotocin (STZ, 40mg/kg). After 21days from the injection of STZ, we induced one full-thickness skin wound in each of the diabetic rats. We randomly divided the rats into four groups: i) placebo; ii) pulsed wave laser (890nm, 80Hz, 0.324J/cm2); iii) metformin; and iv) laser+metformin. Rats received daily intraperitoneal injections of metformin (50mg/kg). On days 7and 15 we inspected the microbial flora of each wound. On day 15 we obtained a standard sample from each healing wound for biomechanical analyses. PBM significantly decreased colony-forming units (CFUs) 7days after wound infliction compared to the placebo group (LSD test, p=0.012). Metformin significantly enhanced the biomechanical property (stress high load) of the wounds compared to the placebo group (LSD test, p=0.028). We observed the same significant result for PBM compared to the placebo group (LSD test, p=0.047). PBM significantly accelerated the wound healing process and significantly reduced CFUs of bacteria in a non-genetic rat model of TII DM.
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Full text: 1 Database: MEDLINE Therapeutic Methods and Therapies TCIM: Terapias_energeticas Main subject: Skin / Wound Healing / Low-Level Light Therapy / Diabetes Mellitus, Type 2 / Metformin Type of study: Prognostic_studies Language: En Journal: J Photochem Photobiol B Year: 2017 Type: Article

Full text: 1 Database: MEDLINE Therapeutic Methods and Therapies TCIM: Terapias_energeticas Main subject: Skin / Wound Healing / Low-Level Light Therapy / Diabetes Mellitus, Type 2 / Metformin Type of study: Prognostic_studies Language: En Journal: J Photochem Photobiol B Year: 2017 Type: Article