Selenomethionine Alleviates AFB1-Induced Damage in Primary Chicken Hepatocytes by Inhibiting CYP450 1A5 Expression via Upregulated SelW Expression.
J Agric Food Chem
; 65(12): 2495-2502, 2017 Mar 29.
Article
in En
| MEDLINE
| ID: mdl-28285519
ABSTRACT
This study aims to evaluate the protective effects of selenomethionine (SeMet) on aflatoxin B1 (AFB1)-induced hepatotoxicity in primary chicken hepatocytes. Cell viability and lactic dehydrogenase activity assays revealed the dose dependence of AFB1 toxicity to chicken hepatocytes. AFB1 concentrations of >0.05 µg/mL significantly reduced glutathione and total superoxide dismutase levels and increased the malondialdehyde concentration and cytochrome P450 enzyme 1A5 (CYP450 1A5) mRNA levels (P < 0.05). AFB1, however, did not affect CYP450 3A37 mRNA levels. Supplementation with 2 µM SeMet protected against AFB1-induced changes and significantly increased selenoprotein W (SelW) mRNA levels (P < 0.05). Additionally, SelW knockdown attenuated the protective effect of SeMet on AFB1-induced damage and significantly increased the level of CYP450 1A5 expression (P < 0.05). Therefore, SeMet alleviates AFB1-induced damage in primary chicken hepatocytes by improving SelW expression, thus inhibiting CYP450 1A5 expression.
Key words
Full text:
1
Database:
MEDLINE
Main subject:
Selenomethionine
/
Aflatoxin B1
/
Hepatocytes
/
Cytochrome P-450 Enzyme System
/
Avian Proteins
/
Selenoprotein W
/
Cytochrome P-450 Enzyme Inhibitors
Language:
En
Journal:
J Agric Food Chem
Year:
2017
Type:
Article