Quantitation of camellianin A in HepG2 cells using a high performance liquid chromatography-electrospray ionization tandem mass spectrometric method.
Chin J Nat Med
; 15(3): 234-240, 2017 Mar.
Article
in En
| MEDLINE
| ID: mdl-28411692
ABSTRACT
The present study was designed to develop a sensitive and selective high performance liquid chromatography-tandem mass spectrometric method for the determination of Camellianin A in HepG2 cells. The extraction of Camellianin A was achieved using 15% trichloroacetic acid and then separated on a C18 column interfaced with a triple quadrupole tandem mass spectrometer in multiple reaction monitoring mode. The mobile phase was consisted of methanol-water (0.1% formic acid) (55 45, V/V). The total run time was 5.0 min. The method was linear in the concentration range of 0.25-250.0 ng·mL-1. The lower limit of quantification was 0.25 ng·mL-1. The intra- and inter-day relative standard deviations of entire concentration range were less than 9.3%. The proposed HPLC-MS/MS method was successfully applied to detect the intracellular concentration of Camellianin A in HepG2 cells.
Key words
Full text:
1
Database:
MEDLINE
Main subject:
Flavonoids
/
Chromatography, High Pressure Liquid
Type of study:
Evaluation_studies
Language:
En
Journal:
Chin J Nat Med
Year:
2017
Type:
Article
Affiliation country:
China