Cucurbita pepo leaf extract induced synthesis of zinc oxide nanoparticles, characterization for the treatment of femoral fracture.
J Photochem Photobiol B
; 195: 12-16, 2019 Jun.
Article
in En
| MEDLINE
| ID: mdl-31029913
ABSTRACT
The present work showed the green, eco-friendly synthesis of zinc oxide nanoparticles (ZnO NPs) using leaf extract of Cucurbita pepo. The prepared ZnO NPs were studied using different characterization techniques such as Fourier transform infrared (FTIR), X-ray diffraction (XRD), UV-Visible spectroscopy (UV-Vis) and Transmission electron microscopy (TEM). TEM images confirmed the formation of NPs with average size of 8â¯nm. XRD showed the crystalline nature of prepared ZnO NPs. This shows that the ZnO NPs induced cytotoxicity that affected the proliferation of MG63 osteoblast-like cells in a concentration dependent manner. Further, the ZnO NPs induced reduction in cell proliferation was confirmed by Fluorescein diacetate hydrolysis (FDA) staining. FDA staining results showed a reduction in fluorescence intensity with an increase in the concentration of ZnO NPs. The untreated control which was unaffected showed the highest fluorescence compared to ZnO NPs treated samples. A significantly diminished fluorescence was observed in samples incubated with 80â¯ppm ZnO NPs. In conclusion, these cell experimental results confirmed that the fabricated ZnO NPs are an important substitute for osteoporotic and bone tissue formation.
Key words
Full text:
1
Database:
MEDLINE
Therapeutic Methods and Therapies TCIM:
Terapias_biologicas
Main subject:
Zinc Oxide
/
Plant Extracts
/
Cucurbita
/
Metal Nanoparticles
Language:
En
Journal:
J Photochem Photobiol B
Year:
2019
Type:
Article