Effect of Celastrus orbiculatus in inhibiting Helicobacter pylori induced inflammatory response by regulating epithelial mesenchymal transition and targeting miR-21/PDCD4 signaling pathway in gastric epithelial cells.
BMC Complement Altern Med
; 19(1): 91, 2019 Apr 29.
Article
in En
| MEDLINE
| ID: mdl-31035975
ABSTRACT
BACKGROUND:
The extract of Celastrus orbiculatus (COE) have been studied for anti-Helicobacter pylori (H. pylori) activity and anti-cancer effects in vitro and in vivo. However, the molecular mechanism by which COE inhibits H. pylori-induced inflammatory response has not been fully elucidated so far.METHODS:
The effects of COE on viability, morphological changes, inflammatory cytokine secretion, protein and mRNA expression were analyzed by MTT assay, enzyme-linked immunosorbent assay (ELISA), immunofluorescence, western blot and real-time PCR (RT-PCR), respectively. The methylation level of programmed cell death 4 (PDCD4) promoter was investigated by methylation-specific PCR. (MSP) .RESULTS:
COE effectively inhibited the H.pylori-induced inflammatory response by regulating epithelial-mesenchymal transition (EMT). The methylation level of PDCD4 promoter was suppressed by COE, which increased the expression ofPDCD4. Moreover, COE could inhibit microRNA-21 (miR-21) expression, as shown by an enhancement of its target gene PDCD4. Furthermore, both miR-21 over-expression and PDCD4 silencing attenuated the anti-inflammatory effect. of COE.CONCLUSIONS:
COE inhibits H. pylori induced inflammatory response through regulating EMT, correlating with inhibition of miR-21/PDCD4 signal pathways in gastric epithelial cells.Key words
Full text:
1
Database:
MEDLINE
Main subject:
Plant Extracts
/
Helicobacter pylori
/
RNA-Binding Proteins
/
Celastrus
/
MicroRNAs
/
Apoptosis Regulatory Proteins
/
Epithelial-Mesenchymal Transition
Language:
En
Journal:
BMC Complement Altern Med
Year:
2019
Type:
Article
Affiliation country:
China