The first method for determination of lipoyllysine in human urine after oral lipoic acid supplementation.
Bioanalysis
; 11(14): 1359-1373, 2019 Jul.
Article
in En
| MEDLINE
| ID: mdl-31368790
ABSTRACT
Aim:
The first method on urinary excreted amounts of lipoyllysine (LLys) after lipoic acid (LA) supplementation was developed and validated. The suggested procedure allowed simultaneous determination of LLys and LA. Methodology &results:
After the conversion of analytes into their reduced forms with tris(2-carboxyethyl)phosphine and derivatization via thiol group with 1-benzyl-2-chloropyridinium bromide, separation of analytes derivatives was performed on C18 column using a gradient mobile phase consisting of acetic acid and acetonitrile. The calibration curves for LA and LLys were linear (R2 > 0.999) in the range of 0.4-12 µM concentration and all validation results were acceptable, according to the US FDA bioanalytical method guidelines.Conclusion:
This method was effectively applied for LA and LLys quantification in human urine after oral LA supplementation.Key words
Full text:
1
Database:
MEDLINE
Main subject:
Thioctic Acid
/
Urinalysis
/
Dietary Supplements
/
Lysine
Language:
En
Journal:
Bioanalysis
Year:
2019
Type:
Article
Affiliation country:
Poland