Linked-read sequencing of gametes allows efficient genome-wide analysis of meiotic recombination.
Nat Commun
; 10(1): 4310, 2019 09 20.
Article
in En
| MEDLINE
| ID: mdl-31541084
ABSTRACT
Meiotic crossovers (COs) ensure proper chromosome segregation and redistribute the genetic variation that is transmitted to the next generation. Large populations and the demand for genome-wide, fine-scale resolution challenge existing methods for CO identification. Taking advantage of linked-read sequencing, we develop a highly efficient method for genome-wide identification of COs at kilobase resolution in pooled recombinants. We first test this method using a pool of Arabidopsis F2 recombinants, and recapitulate results obtained from the same plants using individual whole-genome sequencing. By applying this method to a pool of pollen DNA from an F1 plant, we establish a highly accurate CO landscape without generating or sequencing a single recombinant plant. The simplicity of this approach enables the simultaneous generation and analysis of multiple CO landscapes, accelerating the pace at which mechanisms for the regulation of recombination can be elucidated through efficient comparisons of genotypic and environmental effects on recombination.
Full text:
1
Database:
MEDLINE
Main subject:
Recombination, Genetic
/
Genome, Plant
/
Genotyping Techniques
/
Homologous Recombination
/
Germ Cells
Language:
En
Journal:
Nat Commun
Year:
2019
Type:
Article
Affiliation country:
Germany