Extracellular IL-37 promotes osteogenic differentiation of human bone marrow mesenchymal stem cells via activation of the PI3K/AKT signaling pathway.
Cell Death Dis
; 10(10): 753, 2019 10 03.
Article
in En
| MEDLINE
| ID: mdl-31582734
ABSTRACT
Interleukin (IL)-37, a pivotal anti-inflammatory cytokine and a fundamental inhibitor of innate immunity, has recently been shown to be abnormally expressed in several autoimmune-related orthopedic diseases, including rheumatoid arthritis, ankylosing spondylitis, and osteoporosis. However, the role of IL-37 during osteogenic differentiation of mesenchymal stem cells (MSCs) remains largely unknown. In this study, extracellular IL-37 significantly increased osteoblast-specific gene expression, the number of mineral deposits, and alkaline phosphatase activity of MSCs. Moreover, a signaling pathway was activated in the presence of IL-37. The enhanced osteogenic differentiation of MSCs due to supplementation of IL-37 was partially rescued by the presence of a PI3K/AKT signaling inhibitor. Using a rat calvarial bone defect model, IL-37 significantly improved bone healing. Collectively, these findings indicate that extracellular IL-37 enhanced osteogenesis of MSCs, at least in part by activation of the PI3K/AKT signaling pathway.
Full text:
1
Database:
MEDLINE
Main subject:
Osteogenesis
/
Cell Differentiation
/
Interleukin-1
/
Phosphatidylinositol 3-Kinases
/
Proto-Oncogene Proteins c-akt
/
Extracellular Space
/
Mesenchymal Stem Cells
Type of study:
Prognostic_studies
Language:
En
Journal:
Cell Death Dis
Year:
2019
Type:
Article
Affiliation country:
China