Blueberry anthocyaninenriched extract ameliorates transverse aortic constrictioninduced myocardial dysfunction via the DDAH1/ADMA/NO signaling pathway in mice.
Mol Med Rep
; 21(1): 454-462, 2020 01.
Article
in En
| MEDLINE
| ID: mdl-31746378
ABSTRACT
Blueberry anthocyaninenriched extract (BAE) has been demonstrated to protect against cardiovascular diseases by activating multiple target genes. The present study investigated the effects of BAE on transverse aortic constriction (TAC)induced myocardial dysfunction in mice and explored its possible molecular mechanisms. A total of 30 male mice were divided randomly into control, TAC and TAC + BAE groups. Mice in the TAC + BAE groups were administered BAE by oral gavage for 6 consecutive weeks. Myocardial dysfunction was assessed using echocardiogram, histopathology, TUNEL assay, immunofluorescence staining, reverse transcriptionquantitative PCR and western blot analysis. The results demonstrated that BAE treatment significantly ameliorated heart weight, left ventricular weight, myocardial dysfunction, left ventricular hypertrophy and fibrosis. In addition, BAE treatment alleviated TACinduced inflammation, oxidative stress and apoptosis. Notably, BAE treatment markedly reduced asymmetric dimethylarginine (ADMA) concentration and significantly increased dimethylarginine dimethylaminohydrolase 1 (DDAH1) expression and nitric oxide (NO) production. The present data indicated that BAE treatment ameliorated TACinduced myocardial dysfunction, oxidative stress, inflammatory response and apoptosis via the DDAH1/ADMA/NO signaling pathway.
Key words
Full text:
1
Database:
MEDLINE
Main subject:
Aortic Valve Stenosis
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Cardiovascular Diseases
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Blueberry Plants
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Amidohydrolases
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Nitric Oxide
Language:
En
Journal:
Mol Med Rep
Year:
2020
Type:
Article