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Spatiotemporal Characterizations of Spontaneously Beating Cardiomyocytes with Adaptive Reference Digital Image Correlation.
Shradhanjali, Akankshya; Riehl, Brandon D; Duan, Bin; Yang, Ruiguo; Lim, Jung Yul.
Affiliation
  • Shradhanjali A; Department of Mechanical and Materials Engineering, University of Nebraska-Lincoln, Lincoln, NE, 68588, USA.
  • Riehl BD; Department of Mechanical and Materials Engineering, University of Nebraska-Lincoln, Lincoln, NE, 68588, USA.
  • Duan B; Department of Mechanical and Materials Engineering, University of Nebraska-Lincoln, Lincoln, NE, 68588, USA.
  • Yang R; Mary & Dick Holland Regenerative Medicine Program, University of Nebraska Medical Center, Omaha, NE, 68198, USA.
  • Lim JY; Division of Cardiology, Department of Internal Medicine, University of Nebraska Medical Center, Omaha, NE, 68198, USA.
Sci Rep ; 9(1): 18382, 2019 12 05.
Article in En | MEDLINE | ID: mdl-31804542
We developed an Adaptive Reference-Digital Image Correlation (AR-DIC) method that enables unbiased and accurate mechanics measurements of moving biological tissue samples. We applied the AR-DIC analysis to a spontaneously beating cardiomyocyte (CM) tissue, and could provide correct quantifications of tissue displacement and strain for the beating CMs utilizing physiologically-relevant, sarcomere displacement length-based contraction criteria. The data were further synthesized into novel spatiotemporal parameters of CM contraction to account for the CM beating homogeneity, synchronicity, and propagation as holistic measures of functional myocardial tissue development. Our AR-DIC analyses may thus provide advanced non-invasive characterization tools for assessing the development of spontaneously contracting CMs, suggesting an applicability in myocardial regenerative medicine.
Subject(s)

Full text: 1 Database: MEDLINE Main subject: Neoplastic Stem Cells / Myocytes, Cardiac / Embryonic Stem Cells / Time-Lapse Imaging Type of study: Prognostic_studies Language: En Journal: Sci Rep Year: 2019 Type: Article Affiliation country: United States

Full text: 1 Database: MEDLINE Main subject: Neoplastic Stem Cells / Myocytes, Cardiac / Embryonic Stem Cells / Time-Lapse Imaging Type of study: Prognostic_studies Language: En Journal: Sci Rep Year: 2019 Type: Article Affiliation country: United States