Combined therapy of photobiomodulation and adipose-derived stem cells synergistically improve healing in an ischemic, infected and delayed healing wound model in rats with type 1 diabetes mellitus.
BMJ Open Diabetes Res Care
; 8(1)2020 02.
Article
in En
| MEDLINE
| ID: mdl-32098898
ABSTRACT
OBJECTIVE:
We assessed the therapeutic effects of photobiomodulation (PBM) and adipose-derived stem cell (ADS) treatments individually and together on the maturation step of repairing of a delayed healing wound model in rats with type 1 diabetes mellitus (DM1). RESEARCH DESIGN ANDMETHODS:
We randomly assigned 24 rats with DM1 to four groups (n=6 per group). Group 1 was the control (placebo) group. In group 2, allograft human ADSs were transplanted. Group 3 was subjected to PBM (wavelength 890 nm, peak power output 80 W, pulse frequency 80 Hz, pulsed duration 180 ns, duration of exposure for each point 200 s, power density 0.001 W/cm2, energy density 0.2 J/cm2) immediately after surgery, which continued for 6 days per week for 16 days. Group 4 received both the human ADS and PBM. In addition, we inflicted an ischemic, delayed healing, and infected wound simulation in all of the rats. The wounds were infected with methicillin-resistant Staphylococcus aureus (MRSA).RESULTS:
All three treatment regimens significantly decreased the amount of microbial flora, significantly increased wound strength and significantly modulated inflammatory response and significantly increased angiogenesis on day 16. Microbiological analysis showed that PBM+ADS was significantly better than PBM and ADS alone. In terms of wound closure rate and angiogenesis, PBM+ADS was significantly better than the PBM, ADS and control groups.CONCLUSIONS:
Combination therapy of PBM+ADS is more effective that either PBM or ADS in stimulating skin injury repair, and modulating inflammatory response in an MRSA-infected wound model of rats with DM1.Key words
Full text:
1
Database:
MEDLINE
Therapeutic Methods and Therapies TCIM:
Terapias_energeticas
Main subject:
Wound Healing
/
Wound Infection
/
Adipose Tissue
/
Low-Level Light Therapy
/
Stem Cell Transplantation
/
Diabetes Mellitus, Experimental
/
Diabetes Mellitus, Type 1
/
Methicillin-Resistant Staphylococcus aureus
/
Ischemia
Type of study:
Clinical_trials
Language:
En
Journal:
BMJ Open Diabetes Res Care
Year:
2020
Type:
Article
Affiliation country:
Iran