Use of Microspore-Derived Calli as Explants for Biolistic Transformation of Common Wheat.
Methods Mol Biol
; 2124: 263-279, 2020.
Article
in En
| MEDLINE
| ID: mdl-32277459
There are specific advantages of using microspores as explants: (1) A small number of explant donors are required to obtain the desired number of pollen embryoids for genetic transformation and (2) microspores constitute a synchronous mass of haploid cells, which are transformable by various means and convertible to doubled haploids therefore allow production of homozygous genotypes in a single generation. Additionally, it has been demonstrated in wheat and other crops that microspores can be easily induced to produce embryoids and biolistic approach to produce a large number of transformants. In view of these listed advantages, we optimized the use of microspore-derived calli for biolistic transformation of wheat. The procedure takes about 6 months to obtain the viable transformants in the spring wheat background. In the present communication, we demonstrated the use of this method to produce the reduced immunogenicity wheat genotypes.
Key words
Full text:
1
Database:
MEDLINE
Main subject:
Pollen
/
Transformation, Genetic
/
Triticum
/
Biolistics
Language:
En
Journal:
Methods Mol Biol
Year:
2020
Type:
Article
Affiliation country:
United States