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[Factors Influencing Mononuclear Cell Collection in Tumor Cell Biotherapy].
Jiang, Ying; Li, Ling-Ling; Chi, Hong-Xu; Qiu, Xue-De; Yao, Huan; Tian, Jing; Lin, Jie; Zhang, Lei-Ying; Liu, Xiao-Min; Yu, Yang; Wang, De-Qing.
Affiliation
  • Jiang Y; Department of Transfusion, The First Medical Center of the Chinese PLA General Hospital, Beijing 100853,china.
  • Li LL; Department of Transfusion, The First Medical Center of the Chinese PLA General Hospital, Beijing 100853,china.
  • Chi HX; Department of Transfusion, The First Medical Center of the Chinese PLA General Hospital, Beijing 100853,china.
  • Qiu XD; Department of Transfusion, The First Medical Center of the Chinese PLA General Hospital, Beijing 100853,china.
  • Yao H; Department of Transfusion, The First Medical Center of the Chinese PLA General Hospital, Beijing 100853,china.
  • Tian J; Department of Transfusion, The First Medical Center of the Chinese PLA General Hospital, Beijing 100853,china.
  • Lin J; Department of Transfusion, The First Medical Center of the Chinese PLA General Hospital, Beijing 100853,china.
  • Zhang LY; Department of Transfusion, The First Medical Center of the Chinese PLA General Hospital, Beijing 100853,china.
  • Liu XM; Department of Transfusion, The First Medical Center of the Chinese PLA General Hospital, Beijing 100853,china.
  • Yu Y; Department of Transfusion, The First Medical Center of the Chinese PLA General Hospital, Beijing 100853,china,E-mail: yuyangpla301@163.com.
  • Wang DQ; Department of Transfusion, The First Medical Center of the Chinese PLA General Hospital, Beijing 100853,china,E-mail:deqingw@vip.sina.com.
Zhongguo Shi Yan Xue Ye Xue Za Zhi ; 28(2): 622-628, 2020 Apr.
Article in Zh | MEDLINE | ID: mdl-32319406
OBJECTIVE: To investigate the factors affecting counting and collection efficiency of the final product- mononuclear cells (MNCs) in the collection of mononuclear cells for tumor cell biotherapy. METHODS: The collected data of 142 tumor patients and healthy donors were analyzed, including age, sex, height, weight, BMI, the total blood volume, diagnostic category, vascular access, operator, final product volume, ACD anticoagulant usage, flow rate and circulation times, pre-apheresis Hb, RBC, Plt, WBC, lymphocyte count, monocyte count, neutrophil count, circulating blood volume without anticoagulant, final product MNC and collection efficiency of MNC. CE(collection efficiency)%= final product MNC×100/(pre-apheresis MNC×circulating blood volume without anticoagulant). The factors affecting final products MNC and CE of MNC were detected by T test and multiple linear regression analysis. RESULTS: The CE of tumor patients was higher than that of healthy donors (24.41±1.91,vs 20.01±0.99),(P=0.043), and CE of MNC was different among different operators (P=0.01, H=18.59). There was a positive correlation of the final MNC with the volume of final product, ACD anticoagulant usage and pre-apheresis lymphocyte count (P= 0.00, P= 0.01, P= 0.00, r=0.811); CE of MNC negatively correlated with flow rate and pre-apheresis RBC, but positively correlated with operator's working age and ACD anticoagulant usage (P=0.01, P=0.04, P=0.03, P= 0.00, r=0.495). CONCLUSION: more higher pre-apheresis lymphocyte , more amount of the final product and ACD anticoagulant usage, and more high the final MNC. During the collecting process, more ACD anticoagulant usage and more high operator's seniority, lead to the higher MNC'S CE; while more high pre-apheresis RBC and more fast flow rate, cause the lower the CE of MNC.
Subject(s)

Full text: 1 Database: MEDLINE Main subject: Biological Therapy / Blood Component Removal Language: Zh Journal: Zhongguo Shi Yan Xue Ye Xue Za Zhi Year: 2020 Type: Article Affiliation country: China

Full text: 1 Database: MEDLINE Main subject: Biological Therapy / Blood Component Removal Language: Zh Journal: Zhongguo Shi Yan Xue Ye Xue Za Zhi Year: 2020 Type: Article Affiliation country: China