Structural elucidation and osteogenic activity of a novel heteropolysaccharide from Alhagi pseudalhagi.
Int J Biol Macromol
; 171: 185-197, 2021 Feb 28.
Article
in En
| MEDLINE
| ID: mdl-33412197
ABSTRACT
Alhagi pseudalhagi, commonly known as camel thorn, is used as an indigenous medicinal plant in China. The present study was designed to elucidate the structure of a novel polysaccharide, APP90-2, isolated from Alhagi pseudalhagi and evaluate its osteogenic activity. A homogeneous polysaccharide (APP90-2) was obtained from A. pseudalhagi via DEAE-52 and Sephacryl S-100 columns, with a molecular weight of 5.9 kDa. Monosaccharide, GC-MS, and NMR analyses showed that APP90-2 consisted of α-l-Rhap-(1â, â3)-α-l-Araf-(1â, â5)-α-l-Araf-(1â, â4)-ß-d-Xylp-(1â, α-d-Glcp-(1â, â3,5)-α-l-Araf-(1â, â4)-ß-d-GlcAp-(1â, â4)-3-OAc-α-d-Glcp-(1â, â3)-α-d-Galp-(1â, â3)-ß-d-GalAp-(1â, â4)-α-d-Galp-(1â, â6)-α-d-Manp-(1â, â4,6)-ß-d-Galp-(1â, and â3,6)-ß-d-Glcp-(1â with relative molar ratios of 4.11.86.16.71.71.01.52.72.41.12.32.61.42.0. Morphological analyses revealed that APP90-2 interacted with Congo-red and had an obvious honeycomb structure. Additionally, APP90-2 significantly promoted proliferation, differentiation, and mineralization of MC3T3-E1 cells, indicating that APP90-2 exhibited pronounced osteogenic activity. Therefore, our findings suggest that A. pseudalhagi may be used as an alternative medicine or health supplement for the prevention and treatment of osteoporosis.
Key words
Full text:
1
Database:
MEDLINE
Therapeutic Methods and Therapies TCIM:
Terapias_biologicas
Main subject:
Osteoblasts
/
Osteogenesis
/
Polysaccharides
/
Calcification, Physiologic
/
Bone Density Conservation Agents
/
Fabaceae
Country/Region as subject:
Asia
Language:
En
Journal:
Int J Biol Macromol
Year:
2021
Type:
Article
Affiliation country:
China