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Nigella sativa oil inhibits proliferation and stimulates apoptosis of human lymphocytes in vitro.
Ciesielska-Figlon, Klaudia; Lisowska, Katarzyna A; Mikosik-Roczynska, Anna; Witkowski, Jacek M.
Affiliation
  • Ciesielska-Figlon K; Department of Pathophysiology, Faculty of Medicine, Medical University of Gdansk, Poland.
  • Lisowska KA; Department of Pathophysiology, Faculty of Medicine, Medical University of Gdansk, Poland. Electronic address: katarzyna.lisowska@gumed.edu.pl.
  • Mikosik-Roczynska A; Department of Pathophysiology, Faculty of Medicine, Medical University of Gdansk, Poland.
  • Witkowski JM; Department of Pathophysiology, Faculty of Medicine, Medical University of Gdansk, Poland.
Hum Immunol ; 82(8): 608-614, 2021 Aug.
Article in En | MEDLINE | ID: mdl-33975717
The study aimed to examine the in vitro influence of Nigella sativa oil on human lymphocytes. Cells were stimulated with a monoclonal anti-CD3 antibody in the presence of serial oil ethanol dilutions. Then their proliferation and apoptosis rates were assessed using flow cytometry. Our results demonstrate that the lowest dilutions (1:1 and 1:10) of Nigella sativa oil inhibited lymphocytes' proliferation. The number of cell divisions was 8, 1.25, 1.88 after stimulation with anti-CD3, or its combination with 1:1 and 1:10 oil dilution. The percentage of proliferating cells was 92.48%, 8.75%, 24.3% after stimulation with anti-CD3 antibody, or its combination with 1:1 and 1:10 oil dilution. The mean percentage of living cells was 81% after stimulation with anti-CD3, 13.6%, 19.9% in the presence of 1:1 and 1:10 oil dilution. The preliminary studies show that black seed oil has a potent antiproliferative and proapoptotic effect on human lymphocytes in vitro.
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Full text: 1 Database: MEDLINE Main subject: Plant Oils / Lymphocyte Activation / Lymphocytes / Apoptosis Language: En Journal: Hum Immunol Year: 2021 Type: Article Affiliation country: Poland

Full text: 1 Database: MEDLINE Main subject: Plant Oils / Lymphocyte Activation / Lymphocytes / Apoptosis Language: En Journal: Hum Immunol Year: 2021 Type: Article Affiliation country: Poland