Total Flavonoids of Engelhardia roxburghiana Wall. Leaves Alleviated Foam Cells Formation through AKT/mTOR-Mediated Autophagy in the Progression of Atherosclerosis.
Chem Biodivers
; 18(9): e2100308, 2021 Sep.
Article
in En
| MEDLINE
| ID: mdl-34259387
ABSTRACT
Engelhardia roxburghiana Wall. is a traditional Chinese medicine used for treating cardiovascular diseases. Our previous study has implicated potential effects of total flavonoids of Engelhardia roxburghiana Wall. (TFER) against hyperlipidemia. The aim of the study is to uncover the effects and underlying mechanisms of TFER on foam cells formation after atherosclerosis. We used high fat diet (HFD) induced Apoe-/- mice and oxidized density lipoprotein (ox-LDL) induced THP-1 cells to mimic process of atherosclerosis inâ
vivo and inâ
vitro, respectively. Lipid accumulation, inflammation response, autophagosomes formation and expressions of autophagy related target genes were assessed. Our present study demonstrated TFER (500â
mg/kg) alleviated macrophage infiltration and lipid accumulation in thoracic aortas of HFD-treated mice. In ox-LDL-treated THP-1â
cells, MDC staining and Western blot analysis all indicated that the TFER (200â
µg/ml) reduced foam cells formation and IL-1ß releasing, activated autophagy through suppressing AKT/mTOR signaling, significantly regulating expressions of AKT, p-AKT, mTOR, p-mTOR, Beclinâ
1, LC3-II, p62. It is suggested that TFER alleviated atherosclerosis progression inâ
vivo and inâ
vitro through reducing foam cells formation and inflammatory responses, and the possible mechanism may be due to the activation of macrophage autophagy by inhibiting AKT and mTOR phosphorylation.
Key words
Full text:
1
Database:
MEDLINE
Traditional Medicines:
Medicinas_tradicionales_de_asia
/
Medicina_china
Therapeutic Methods and Therapies TCIM:
Terapias_biologicas
Main subject:
Autophagy
/
Flavonoids
/
Drugs, Chinese Herbal
/
Plant Leaves
/
Juglandaceae
/
Atherosclerosis
Type of study:
Diagnostic_studies
Language:
En
Journal:
Chem Biodivers
Year:
2021
Type:
Article