ALT, alanine aminotransferase; AST, aspartate aminotransferase; BCRJ, Cell bank of Rio de Janeiro; BFS, bovine fetal serum; Caspase-8; DARP, dopamine releasing protein; DMEM, Dulbecco's Modified Eagle's Medium; DMSO, dimethyl sulfoxide; DPPH, 2,2-diphenyl-1-picrylhydrazyl; EDTA, ethylenediaminetetraacetic; EEEp, ethanol extract of Eleutherine plicata; Eleutherin; Eleutherine plicata; Eleutherol; FADD, Fas associated death domain; FDMEp, dichloromethane fraction of Eleutherine plicata; FrAE, ethyl acetate fraction of Elutherine plicata; GA, Genetic Algorithm; GOLD, Genetic Optimization for Ligand Docking; HPLC, high performance liquid chromatography; IC50, 50 % cytotoxic concentration; Isoeleutherin; MD, molecular dynamics; MTT, ([3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide]); NMR, nuclear magnetic resonance; NMU, N-methyl-N-nitrosurea; OECD, Organization for Economic Co-Operation and Development; PDB, Protein Data Bank; ROS, reactive oxygen species; RPMI, Roswell Park Memorial Institute medium; RSMD, root mean square deviation; TLC, tin layer chromatography; TNFR, tumour necrosis fator receptor; Toxicity; rpm, rotations per minute