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Novel suction-based in vivo cutaneous DNA transfection platform.
Lallow, Emran O; Jhumur, Nandita C; Ahmed, Ijaz; Kudchodkar, Sagar B; Roberts, Christine C; Jeong, Moonsup; Melnik, Juliet M; Park, Sarah H; Muthumani, Kar; Shan, Jerry W; Zahn, Jeffrey D; Shreiber, David I; Singer, Jonathan P; Park, Young K; Maslow, Joel N; Lin, Hao.
Affiliation
  • Lallow EO; Department of Mechanical and Aerospace Engineering, Rutgers, The State University of New Jersey, Piscataway, NJ 08854, USA.
  • Jhumur NC; Department of Mechanical and Aerospace Engineering, Rutgers, The State University of New Jersey, Piscataway, NJ 08854, USA.
  • Ahmed I; Department of Biomedical Engineering, Rutgers, The State University of New Jersey, Piscataway, NJ 08854, USA.
  • Kudchodkar SB; GeneOne Life Science, Seoul, South Korea.
  • Roberts CC; GeneOne Life Science, Seoul, South Korea.
  • Jeong M; GeneOne Life Science, Seoul, South Korea.
  • Melnik JM; Graduate School of Biomedical Sciences, Rutgers, The State University of New Jersey, Piscataway, NJ 08854, USA.
  • Park SH; Department of Mechanical and Aerospace Engineering, Rutgers, The State University of New Jersey, Piscataway, NJ 08854, USA.
  • Muthumani K; GeneOne Life Science, Seoul, South Korea.
  • Shan JW; Department of Mechanical and Aerospace Engineering, Rutgers, The State University of New Jersey, Piscataway, NJ 08854, USA.
  • Zahn JD; Department of Biomedical Engineering, Rutgers, The State University of New Jersey, Piscataway, NJ 08854, USA.
  • Shreiber DI; Department of Biomedical Engineering, Rutgers, The State University of New Jersey, Piscataway, NJ 08854, USA.
  • Singer JP; Department of Mechanical and Aerospace Engineering, Rutgers, The State University of New Jersey, Piscataway, NJ 08854, USA.
  • Park YK; GeneOne Life Science, Seoul, South Korea.
  • Maslow JN; GeneOne Life Science, Seoul, South Korea.
  • Lin H; Department of Mechanical and Aerospace Engineering, Rutgers, The State University of New Jersey, Piscataway, NJ 08854, USA.
Sci Adv ; 7(45): eabj0611, 2021 11 05.
Article in En | MEDLINE | ID: mdl-34739313
ABSTRACT
This work reports a suction-based cutaneous delivery method for in vivo DNA transfection. Following intradermal Mantoux injection of plasmid DNA in a rat model, a moderate negative pressure is applied to the injection site, a technique similar to Chinese báguàn and Middle Eastern hijama cupping therapies. Strong GFP expression was demonstrated with pEGFP-N1 plasmids where fluorescence was observed as early as 1 hour after dosing. Modeling indicates a strong correlation between focal strain/stress and expression patterns. The absence of visible and/or histological tissue injury contrasts with current in vivo transfection systems such as electroporation. Specific utility was demonstrated with a synthetic SARS-CoV-2 DNA vaccine, which generated host humoral immune response in rats with notable antibody production. This method enables an easy-to-use, cost-effective, and highly scalable platform for both laboratorial transfection needs and clinical applications for nucleic acid­based therapeutics and vaccines.
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Full text: 1 Database: MEDLINE Traditional Medicines: Medicinas_tradicionales_de_asia / Medicina_china Main subject: Skin / DNA / Transfection / Vaccines, DNA / COVID-19 Vaccines / SARS-CoV-2 / COVID-19 Language: En Journal: Sci Adv Year: 2021 Type: Article Affiliation country: United States
Fulltext
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Full text: 1 Database: MEDLINE Traditional Medicines: Medicinas_tradicionales_de_asia / Medicina_china Main subject: Skin / DNA / Transfection / Vaccines, DNA / COVID-19 Vaccines / SARS-CoV-2 / COVID-19 Language: En Journal: Sci Adv Year: 2021 Type: Article Affiliation country: United States