BtuB-Dependent Infection of the T5-like Yersinia Phage ÏR2-01.
Viruses
; 13(11)2021 10 28.
Article
in En
| MEDLINE
| ID: mdl-34834977
ABSTRACT
Yersinia enterocolitica is a food-borne Gram-negative pathogen responsible for several gastrointestinal disorders. Host-specific lytic bacteriophages have been increasingly used recently as an alternative or complementary treatment to combat bacterial infections, especially when antibiotics fail. Here, we describe the proteogenomic characterization and host receptor identification of the siphovirus vB_YenS_ÏR2-01 (in short, ÏR2-01) that infects strains of several Yersinia enterocolitica serotypes. The ÏR2-01 genome contains 154 predicted genes, 117 of which encode products that are homologous to those of Escherichia bacteriophage T5. The ÏR2-01 and T5 genomes are largely syntenic, with the major differences residing in areas encoding hypothetical ÏR2-01 proteins. Label-free mass-spectrometry-based proteomics confirmed the expression of 90 of the ÏR2-01 genes, with 88 of these being either phage particle structural or phage-particle-associated proteins. In vitro transposon-based host mutagenesis and ÏR2-01 adsorption experiments identified the outer membrane vitamin B12 receptor BtuB as the host receptor. This study provides a proteogenomic characterization of a T5-type bacteriophage and identifies specific Y. enterocolitica strains sensitive to infection with possible future applications of ÏR2-01 as a food biocontrol or phage therapy agent.
Key words
Full text:
1
Database:
MEDLINE
Main subject:
Membrane Transport Proteins
/
Bacterial Outer Membrane Proteins
/
Bacterial Proteins
/
Yersinia
/
Siphoviridae
Language:
En
Journal:
Viruses
Year:
2021
Type:
Article
Affiliation country:
Sweden